Escherichia virus T5,sometimes calledBacteriophage T5is a caudalviruswithin the familyDemerecviridae.Thisbacteriophagespecifically infectsE. colibacterial cells and follows alytic life cycle.
| Escherichia virus T5 | |
|---|---|
| |
| Bacteriophage T5 Structure at Atomic Resolution[1] | |
Virus classification
| |
| (unranked): | Virus |
| Realm: | Duplodnaviria |
| Kingdom: | Heunggongvirae |
| Phylum: | Uroviricota |
| Class: | Caudoviricetes |
| Order: | Caudovirales |
| Family: | Demerecviridae |
| Genus: | Tequintavirus |
| Species: | Escherichia virus T5
|
| Synonyms[2] | |
| |
Structure and genome
edit
The T5virionincludes a 90 nanometericosahedralcapsid(head) and a 250 nanometer-long flexible, non-contractiletail.[3]
The capsid contains the phage's 121,750 base pair, double-strandedDNAgenomewhich encodes about 168 proteins (now reduced to 162).[4]The genome has a unique sequence of 111,613 bp with two identical large direct terminal repetitions of 10,139 bp. When the genome sequence was published in 2005, only 61 (36.3%) of the 168 encoded proteins had been assigned functions based on homology to known sequences. More than half of all genes (92 or 54.7%) were predicted ORFs lacking similarity to any known proteins.[5]The number of uncharacterized proteins remains high at about 50% of the genome (based on the latest annotation of the reference proteome inUniprot, 2021).[4]
Infection
editBacteriophage T5 has been shown to infectE. coliafter its receptor binding protein, pb5, binds to the host cell's outer membraneferrichrometransporter, FhuA. The binding triggers structural changes in pb5 and eventually leads to DNA release from the phage capsid.[6][7]
References
edit- ^Victor Padilla-Sanchez. (2024). Bacteriophage T5 Structural Model at Atomic Resolution. Zenodo.https://doi.org/10.5281/zenodo.14174057
- ^Krupovic, Mart; et al. (May 2015)."To rename all (522) existing bacterial virus and 2 archaeal virus species"(PDF).International Committee on Taxonomy of Viruses (ICTV).Retrieved27 February2020.
- ^Effantin G. et al.: Bacteriophage T5 structure reveals similarities with HK97 and T4 suggesting evolutionary relationships. J Mol Biol. (2006) 361, 993–1002,doi:10.1016/j.jmb.2006.06.081
- ^ab"Escherichia phage T5 (Enterobacteria phage T5)".www.uniprot.org.Retrieved2021-04-02.
- ^Wang, Jianbin; Jiang, Yan; Vincent, Myriam; Sun, Yongqiao; Yu, Hong; Wang, Jing; Bao, Qiyu; Kong, Huimin; Hu, Songnian (2005-02-05)."Complete genome sequence of bacteriophage T5".Virology.332(1): 45–65.doi:10.1016/j.virol.2004.10.049.ISSN0042-6822.PMID15661140.
- ^Flayhan, A; Wien, F; Paternostre, M; Boulanger, P; Breyton, C (Sep 2012)."New insights into pb5, the receptor binding protein of bacteriophage T5, and its interaction with its Escherichia coli receptor FhuA".Biochimie.94(9): 1982–9.doi:10.1016/j.biochi.2012.05.021.PMID22659573.S2CID20354844.
- ^Basit, H; Shivaji Sharma, K; Van der Heyden, A; Gondran, C; Breyton, C; Dumy, P; Winnik, FM; Labbé, P (May 11, 2012). "Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5".Chemical Communications.48(48): 6037–9.doi:10.1039/c2cc31107k.PMID22576748.S2CID19703515.
External links
edit
 | Thisvirus-related article is astub.You can help Wikipedia byexpanding it. |