Cerebroside

Cerebrosides(monoglycosylceramides) are a group ofglycosphingolipidswhich are important components of animalmuscleandnerve cellmembranes.

General structures ofsphingolipids

They consist of aceramidewith a single sugar residue at the 1-hydroxyl moiety. The sugar residue can be eitherglucoseorgalactose;the two major types are therefore calledglucocerebrosides(a.k.a. glucosylceramides) andgalactocerebrosides(a.k.a. galactosylceramides). Galactocerebrosides are typically found in neural tissue, while glucocerebrosides are found in other tissues.

Structure

The fundamental structure of a cerebroside isceramide.Monoglycosyl and oligoglycosylceramides having a mono orpolysaccharidebonded glycosidically to the terminal OH group of ceramide are defined as cerebrosides. Sphingosine is the main long-chain base present in ceramide.

Galactosylceramide is the principal glycosphingolipid in brain tissue. Galactosylceramides are present in all nervous tissues, and can compose up to 2% dry weight of grey matter and 12% of white matter. They are major constituents ofoligodendrocytes.Glucosylceramide is found at low levels in animal cells such as thespleen,erythrocytes,and nervous tissues, especiallyneurons.Glucosylceramide is a major constituent of skin lipids, where it is essential for lamellar body formation in the stratum corneum and to maintain the water permeability barrier of the skin. Glucosylceramide is the only glycosphingolipid common to plants, fungi and animals. It is usually considered to be the principal glycosphingolipid in plants. It is a major component of the outer layer of the plasma membrane. Galactosylceramides have not been found in plants.

Monogalactosylceramide is the largest single component of themyelin sheathofnerves.Cerebroside synthesis can therefore give a measurement ofmyelinformation or remyelination.The sugar moiety is linked glycosidically to the C-1 hydroxyl group of ceramide, such as in lactosylceramide. Cerebrosides containing a sulfuric ester (sulfate) group, known as sulfatides, also occur in the myelin sheath of nerves. These compounds are preferably named as sulfates of the parent glycosphingolipid.

Synthesis

The biosynthesis of monoglycosylceramides requires a direct transfer of the carbohydrate moiety from a sugar-nucleotide, such as uridine 5-diphosphate(UDP)-galactose, or UDP-glucose to the ceramide unit. The glycosyl-transferase catalyzed reaction results in an inversion of the glycosidic bond stereochemistry, changing from α →β. Synthesis of galactosylceramide, and glucosylceramide occurs on the lumenal surface of the endoplasmic reticulum, and on the cytosolic side of the early Golgi membranes respectively.

Physical properties

The melting point of cerebrosides is considerably greater than physiological body temperature, >37.0 °C, giving glycolipids a paracrystalline, similar to liquid crystal structure. Cerebroside molecules are able form up to eight intermolecular hydrogen bonds between the polar hydrogens of the sugar and the hydroxy and amide groups of the sphingosine base of the ceramide. These hydrogen bonds within the cerebrosides result in the molecules having a high transition temperature and compact alignment. Monoglycosylceramides in conjunction with cholesterol are prevalent in the lipid-raft micro domain, which are important sites in the binding of proteins, and enzyme-receptor interactions.

Catabolism

Degradation of glycosphingolipids occurs in the lysosome, which contains digestive enzymes in animal cells. The lysosome breaks down the glycosphingolipid to its primary components, fatty acids, sphingosine, and saccharide.

Chemical analysis

Analysis of monoglycosylceramides can be done by high-resolution thin-layer chromatography, high-performance liquid chromatography (HPLC), and mass spectrometry. Reversed-phase HPLC is now the standard method for separation of molecular species, often after benzoylation, enabling lipids to be detected by UV spectrophotometry.

Role in disease

A defect in the degradation of glucocerebrosides isGaucher's disease.The corresponding defects for galactocerebrosides are:

a) Ceramide trihexoside (globotriaosylceramide) accumulation –Fabry's disease.Clinical features include acroparaesthesia (tingling, pins and needles sensation in the extremities)

b) Galactocerebroside (galactosylceramidase) accumulation –Krabbe disease.

External links

• Cerebrosidesat the U.S. National Library of MedicineMedical Subject Headings(MeSH)
• The Lipid Library: Monoglycosylceramides