Peridininis a light-harvestingapocarotenoid,apigmentassociated withchlorophylland found in theperidinin-chlorophyll-protein(PCP)light-harvesting complexindinoflagellates,best studied inAmphidinium carterae.[1]
![]() | |
![]() | |
Names | |
---|---|
IUPAC name
Acetic acid [(1S,3R)-3-hydroxy-4-[(3E,5E,7E,9E,11Z)-11-[4-[(E)-2-[(1S,4S,6R)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]heptan-1-yl]vinyl]-5-oxo-2-furylidene]-3,10-dimethylundeca-1,3,5,7,9-pentaenylidene]-3,5,5-trimethylcyclohexyl] ester
| |
Identifiers | |
3D model (JSmol)
|
|
PubChemCID
|
|
CompTox Dashboard(EPA)
|
|
| |
Properties | |
C39H50O7 | |
Molar mass | 630.822g·mol−1 |
Except where otherwise noted, data are given for materials in theirstandard state(at 25 °C [77 °F], 100 kPa).
|
Biological significance
editPeridinin is anapocarotenoidpigmentthat some organisms use inphotosynthesis.Many photosyntheticdinoflagellatesuse peridinin, which absorbs blue-green light in the 470-550nm range, outside the range accessible tochlorophyllmolecules. Theperidinin-chlorophyll-protein complexis a specialized molecular complex consisting of a boat-shaped protein molecule with a large central cavity that contains peridinin, chlorophyll, and lipid molecules, usually in a 4:1 ratio of peridinin to chlorophyll.[1][2][3]
Spectral characteristics
edit- Absorption maximum: 483 nm
- Emission maximum: 676 nm
- Extinction coefficient (ε): 1.96 x 106M−1cm−1
- A483/A280≥ 4.6
Applications
editPeridinin chlorophyll (PerCP) is commonly used inimmunoassayssuch asfluorescence-activated cell sorting(FACS) andflow cytometry.The fluorophore is covalently linked to proteins or antibodies for use in research applications.[4]
References
edit- ^abcHofmann, E; Wrench, PM; Sharples, FP; Hiller, RG; Welte, W; Diederichs, K (21 June 1996)."Structural basis of light harvesting by carotenoids: peridinin-chlorophyll-protein from Amphidinium carterae".Science.272(5269): 1788–91.doi:10.1126/science.272.5269.1788.PMID8650577.
- ^Schulte, Tim; Johanning, Silke; Hofmann, Eckhard (December 2010). "Structure and function of native and refolded peridinin-chlorophyll-proteins from dinoflagellates".European Journal of Cell Biology.89(12): 990–997.doi:10.1016/j.ejcb.2010.08.004.PMID20846743.
- ^Jiang, Jing; Zhang, Hao; Kang, Yisheng; Bina, David; Lo, Cynthia S.;Blankenship, Robert E.(July 2012)."Characterization of the peridinin–chlorophyll a-protein complex in the dinoflagellate Symbiodinium".Biochimica et Biophysica Acta (BBA) - Bioenergetics.1817(7): 983–989.doi:10.1016/j.bbabio.2012.03.027.PMC3947849.PMID22497797.
- ^"Peridinin Chlorophyll (PerCP)".Archived fromthe originalon 2016-04-23.Retrieved2014-06-07.