Wikipedia

Microfluidics

Microfluidicsrefers to a system that manipulates a small amount offluids(10−9to 10−18liters) using small channels with sizes of ten to hundreds of micrometres. It is a multidisciplinary field that involves molecular analysis,molecular biology,andmicroelectronics.[1]It has practical applications in the design of systems that process low volumes of fluids to achievemultiple xing,automation, andhigh-throughput screening.Microfluidics emerged in the beginning of the 1980s and is used in the development ofinkjetprintheads,DNA chips,lab-on-a-chiptechnology, micro-propulsion, and micro-thermal technologies.

Microfluidic device
NIST researchers have combined a glass slide, plastic sheets and double-sided tape to create an inexpensive and simple-to-build microfluidic device for exposing an array of cells to different concentrations of a chemical.

Typically, micro means one of the following features:

  • Small volumes (μL, nL, pL, fL)
  • Small size
  • Low energy consumption
  • Microdomain effects

Typically microfluidic systems transport, mix, separate, or otherwise process fluids. Various applications rely on passive fluid control usingcapillary forces,in the form of capillary flow modifying elements, akin to flow resistors and flow accelerators. In some applications, external actuation means are additionally used for a directed transport of the media. Examples are rotary drives applying centrifugal forces for the fluid transport on the passive chips.Active microfluidicsrefers to the defined manipulation of the working fluid by active (micro) components such asmicropumpsormicrovalves.Micropumps supply fluids in a continuous manner or are used for dosing. Microvalves determine the flow direction or the mode of movement of pumped liquids. Often, processes normally carried out in a lab are miniaturised on a single chip, which enhances efficiency and mobility, and reduces sample and reagent volumes.

Microscale behaviour of fluids

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Silicone rubber and glass microfluidic devices. Top: a photograph of the devices. Bottom:Phase contrastmicrographsof a serpentine channel ~15μmwide.

The behaviour of fluids at the microscale can differ from "macrofluidic" behaviour in that factors such assurface tension,energy dissipation, and fluidic resistance start to dominate the system. Microfluidics studies how these behaviours change, and how they can be worked around, or exploited for new uses.[2][3][4][5][6]

At small scales (channel size of around 100nanometersto 500micrometers) some interesting and sometimes unintuitive properties appear. In particular, theReynolds number(which compares the effect of the momentum of a fluid to the effect ofviscosity) can become very low. A key consequence is co-flowing fluids do not necessarily mix in the traditional sense, as flow becomeslaminarrather thanturbulent;molecular transport between them must often be throughdiffusion.[7]

High specificity of chemical and physical properties (concentration, pH, temperature, shear force, etc.) can also be ensured resulting in more uniform reaction conditions and higher grade products in single and multi-step reactions.[8][9]

Various kinds of microfluidic flows

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Microfluidic flows need only be constrained by geometrical length scale – the modalities and methods used to achieve such a geometrical constraint are highly dependent on the targeted application.[10]Traditionally, microfluidic flows have been generated inside closed channels with the channel cross section being in the order of 10 μm x 10 μm. Each of these methods has its own associated techniques to maintain robust fluid flow which have matured over several years.[citation needed]

Open microfluidics

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The behavior of fluids and their control in open microchannels was pioneered around 2005[11]and applied in air-to-liquid sample collection[12][13]and chromatography.[14]Inopen microfluidics,at least one boundary of the system is removed, exposing the fluid to air or another interface (i.e. liquid).[15][16][17]Advantages of open microfluidics include accessibility to the flowing liquid for intervention, larger liquid-gas surface area, and minimized bubble formation.[18][15][17][19]Another advantage of open microfluidics is the ability to integrate open systems with surface-tension driven fluid flow, which eliminates the need for external pumping methods such as peristaltic or syringe pumps.[20]Open microfluidic devices are also easy and inexpensive to fabricate by milling, thermoforming, and hot em Boss ing.[21][22][23][24]In addition, open microfluidics eliminates the need to glue or bond a cover for devices, which could be detrimental to capillary flows. Examples of open microfluidics include open-channel microfluidics, rail-based microfluidics,paper-based,and thread-based microfluidics.[15][20][25]Disadvantages to open systems include susceptibility to evaporation,[26]contamination,[27]and limited flow rate.[17]

Continuous-flow microfluidics

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Continuous flow microfluidics rely on the control of a steady stateliquid flowthrough narrow channels or porous media predominantly by accelerating or hindering fluid flow in capillary elements.[28]In paper based microfluidics, capillary elements can be achieved through the simple variation of section geometry. In general, the actuation ofliquid flowis implemented either by externalpressuresources, external mechanicalpumps,integrated mechanicalmicropumps,or by combinations of capillary forces andelectrokineticmechanisms.[29][30]Continuous-flow microfluidic operation is the mainstream approach because it is easy to implement and less sensitive to protein fouling problems. Continuous-flow devices are adequate for many well-defined and simple biochemical applications, and for certain tasks such as chemical separation, but they are less suitable for tasks requiring a high degree of flexibility or fluid manipulations. These closed-channel systems are inherently difficult to integrate and scale because the parameters that govern flow field vary along the flow path making the fluid flow at any one location dependent on the properties of the entire system. Permanently etched microstructures also lead to limited reconfigurability and poor fault tolerance capability. Computer-aided design automation approaches for continuous-flow microfluidics have been proposed in recent years to alleviate the design effort and to solve the scalability problems.[31]

micro fluid sensor

Process monitoring capabilities in continuous-flow systems can be achieved with highly sensitive microfluidic flow sensors based onMEMStechnology, which offers resolutions down to the nanoliter range.[32]

Droplet-based microfluidics

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Main article:Droplet-based microfluidics
High frame rate video showing microbubble pinch-off formation in a flow-focusing microfluidic device[33]

Droplet-based microfluidics is a subcategory of microfluidics in contrast with continuous microfluidics; droplet-based microfluidics manipulates discrete volumes of fluids in immiscible phases with low Reynolds number and laminar flow regimes. Interest in droplet-based microfluidics systems has been growing substantially in past decades. Microdroplets allow for handling miniature volumes (μL to fL) of fluids conveniently, provide better mi xing, encapsulation, sorting, and sensing, and suit high throughput experiments.[34]Exploiting the benefits of droplet-based microfluidics efficiently requires a deep understanding of droplet generation[35]to perform various logical operations[36][37]such as droplet manipulation,[38]droplet sorting,[39]droplet merging,[40]and droplet breakup.[41]

Digital microfluidics

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Main article:Digital microfluidics

Alternatives to the above closed-channel continuous-flow systems include novel open structures, where discrete, independently controllable droplets are manipulated on a substrate usingelectrowetting.Following the analogy of digital microelectronics, this approach is referred to asdigital microfluidics.Le Pesant et al. pioneered the use of electrocapillary forces to move droplets on a digital track.[42]The "fluid transistor" pioneered by Cytonix[43]also played a role. The technology was subsequently commercialised by Duke University. By using discrete unit-volume droplets,[35]a microfluidic function can be reduced to a set of repeated basic operations, i.e., moving one unit of fluid over one unit of distance. This "digitisation" method facilitates the use of a hierarchical and cell-based approach for microfluidic biochip design. Therefore, digital microfluidics offers a flexible and scalable system architecture as well as highfault-tolerancecapability. Moreover, because each droplet can be controlled independently, these systems also have dynamic reconfigurability, whereby groups of unit cells in a microfluidic array can be reconfigured to change their functionality during the concurrent execution of a set of bioassays. Although droplets are manipulated in confined microfluidic channels, since the control on droplets is not independent, it should not be confused as "digital microfluidics". One common actuation method for digital microfluidics iselectrowetting-on-dielectric (EWOD).[44]Many lab-on-a-chip applications have been demonstrated within the digital microfluidics paradigm using electrowetting. However, recently other techniques for droplet manipulation have also been demonstrated using magnetic force,[45]surface acoustic waves,[46]optoelectrowetting,mechanical actuation,[47]etc.

Paper-based microfluidics

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Main article:Paper-based microfluidics

Paper-based microfluidic devices fill a growing niche for portable, cheap, and user-friendly medical diagnostic systems.[48] Paper based microfluidics rely on the phenomenon of capillary penetration in porous media.[49]To tune fluid penetration in porous substrates such as paper in two and three dimensions, the pore structure, wettability and geometry of the microfluidic devices can be controlled while the viscosity and evaporation rate of the liquid play a further significant role. Many such devices feature hydrophobic barriers on hydrophilic paper that passively transport aqueous solutions to outlets where biological reactions take place.[50]Paper-based microfluidics are considered as portable point-of-care biosensors used in a remote setting where advanced medical diagnostic tools are not accessible.[51]Current applications include portable glucose detection[52]and environmental testing,[53]with hopes of reaching areas that lack advanced medical diagnostic tools.

Particle detection microfluidics

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One application area that has seen significant academic effort and some commercial effort is in the area of particle detection in fluids. Particle detection of small fluid-borne particles down to about 1 μm in diameter is typically done using aCoulter counter,in which electrical signals are generated when a weakly-conducting fluid such as insaline wateris passed through a small (~100 μm diameter) pore, so that an electrical signal is generated that is directly proportional to the ratio of the particle volume to the pore volume. The physics behind this is relatively simple, described in a classic paper by DeBlois and Bean,[54]and the implementation first described in Coulter's original patent.[55]This is the method used to e.g. size and count erythrocytes (red blood cells) as well as leukocytes (white blood cells) for standard blood analysis. The generic term for this method isresistive pulse sensing(RPS); Coulter counting is a trademark term. However, the RPS method does not work well for particles below 1 μm diameter, as thesignal-to-noise ratiofalls below the reliably detectable limit, set mostly by the size of the pore in which the analyte passes and the input noise of the first-stageamplifier.[citation needed]

The limit on the pore size in traditional RPS Coulter counters is set by the method used to make the pores, which while a trade secret, most likely[according to whom?]uses traditional mechanical methods. This is where microfluidics can have an impact: Thelithography-based production of microfluidic devices, or more likely the production of reusable molds for making microfluidic devices using amoldingprocess, is limited to sizes much smaller than traditionalmachining.Critical dimensions down to 1 μm are easily fabricated, and with a bit more effort and expense, feature sizes below 100 nm can be patterned reliably as well. This enables the inexpensive production of pores integrated in a microfluidic circuit where the pore diameters can reach sizes of order 100 nm, with a concomitant reduction in the minimum particle diameters by several orders of magnitude.

As a result, there has been some university-based development of microfluidic particle counting and sizing[56][57][58]with the accompanying commercialization of this technology. This method has been termed microfluidicresistive pulse sensing(MRPS).

Microfluidic-assisted magnetophoresis

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One major area of application for microfluidic devices is the separation and sorting of different fluids or cell types. Recent developments in the microfluidics field have seen the integration of microfluidic devices withmagnetophoresis:the migration of particles by amagnetic field.[59]This can be accomplished by sending a fluid containing at least one magnetic component through a microfluidic channel that has amagnetpositioned along the length of the channel. This creates a magnetic field inside the microfluidic channel which drawsmagneticallyactive substances towards it, effectively separating the magnetic and non-magnetic components of the fluid. This technique can be readily utilized inindustrialsettings where the fluid at hand already contains magnetically active material. For example, a handful ofmetallic impuritiescan find their way into certain consumable liquids, namelymilkand otherdairyproducts.[60]Conveniently, in the case of milk, many of these metal contaminants exhibitparamagnetism.Therefore, before packaging, milk can be flowed through channels with magnetic gradients as a means of purifying out the metal contaminants.

Other, more research-oriented applications of microfluidic-assisted magnetophoresis are numerous and are generally targeted towardscellseparation. The general way this is accomplished involves several steps. First, a paramagnetic substance (usually micro/nanoparticlesor aparamagnetic fluid)[61]needs to befunctionalizedto target the cell type of interest. This can be accomplished by identifying atransmembranal proteinunique to the cell type of interest and subsequently functionalizing magnetic particles with the complementaryantigenorantibody.[60][62][63][64][65]Once the magnetic particles are functionalized, they are dispersed in a cell mixture where they bind to only the cells of interest. The resulting cell/particle mixture can then be flowed through a microfluidic device with a magnetic field to separate the targeted cells from the rest.

Conversely, microfluidic-assisted magnetophoresis may be used to facilitate efficient mi xing within microdroplets or plugs. To accomplish this, microdroplets are injected with paramagnetic nanoparticles and are flowed through a straight channel which passes through rapidly alternating magnetic fields. This causes the magnetic particles to be quickly pushed from side to side within the droplet and results in the mi xing of the microdroplet contents.[64]This eliminates the need for tedious engineering considerations that are necessary for traditional, channel-based droplet mi xing. Other research has also shown that the label-free separation of cells may be possible by suspending cells in a paramagnetic fluid and taking advantage of the magneto-Archimedes effect.[66][67]While this does eliminate the complexity of particle functionalization, more research is needed to fully understand the magneto-Archimedes phenomenon and how it can be used to this end. This is not an exhaustive list of the various applications of microfluidic-assisted magnetophoresis; the above examples merely highlight the versatility of thisseparation techniquein both current and future applications.

Key application areas

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Microfluidic structures include micropneumatic systems, i.e. microsystems for the handling of off-chip fluids (liquid pumps, gas valves, etc.), and microfluidic structures for the on-chip handling of nanoliter (nl) and picoliter (pl) volumes.[68]To date, the most successful commercial application of microfluidics is theinkjet printhead.[69]Additionally, microfluidic manufacturing advances mean that makers can produce the devices in low-cost plastics[70]and automatically verify part quality.[71]

Advances in microfluidics technology are revolutionizingmolecular biologyprocedures for enzymatic analysis (e.g.,glucoseandlactateassays),DNAanalysis (e.g.,polymerase chain reactionand high-throughputsequencing),proteomics,and in chemical synthesis.[28][72]The basic idea of microfluidic biochips is to integrateassayoperations such as detection, as well as sample pre-treatment and sample preparation on one chip.[73][74]

An emerging application area for biochips isclinical pathology,especially the immediatepoint-of-carediagnosis ofdiseases.[75]In addition, microfluidics-based devices, capable of continuous sampling and real-time testing of air/water samples for biochemicaltoxinsand other dangerouspathogens,[76]can serve as an always-on"bio-smoke alarm"for early warning.

Microfluidic technology has led to the creation of powerful tools for biologists to control the complete cellular environment, leading to new questions and discoveries. Many diverse advantages of this technology for microbiology are listed below:

  • General single cell studies including growth[77][34]
  • Cellular aging: microfluidic devices such as the "mother machine" allow tracking of thousands of individual cells for many generations until they die[77]
  • Microenvironmental control: ranging from mechanical environment[78]to chemical environment[79][80]
  • Precise spatiotemporal concentration gradients by incorporating multiple chemical inputs to a single device[81]
  • Force measurements of adherent cells or confined chromosomes: objects trapped in a microfluidic device can be directly manipulated usingoptical tweezersor other force-generating methods[82]
  • Confining cells and exerting controlled forces by coupling with external force-generation methods such asStokes flow,optical tweezer,or controlled deformation of the PDMS (Polydimethylsiloxane) device[82][83][84]
  • Electric field integration[84]
  • Plant on a chip and plant tissue culture[85]
  • Antibiotic resistance: microfluidic devices can be used as heterogeneous environments for microorganisms. In a heterogeneous environment, it is easier for a microorganism to evolve. This can be useful for testing the acceleration of evolution of a microorganism / for testing the development of antibiotic resistance.
  • Viral fusion: these devices also allow the study of the several steps and conditions required for viruses to bind and enter host cells. Information regarding efficiency, kinetics and specific steps of the binding and fusion processes can be obtained using microfluidic flow cells.[86]

Some of these areas are further elaborated in the sections below:

DNA chips (microarrays)

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Early biochips were based on the idea of aDNA microarray,e.g., the GeneChip DNAarray fromAffymetrix,which is a piece of glass, plastic or silicon substrate, on which pieces of DNA (probes) are affixed in a microscopic array. Similar to aDNA microarray,aprotein arrayis a miniature array where a multitude of different capture agents, most frequently monoclonalantibodies,are deposited on a chip surface; they are used to determine the presence and/or amount ofproteinsin biological samples, e.g.,blood.A drawback ofDNAandprotein arraysis that they are neither reconfigurable norscalableafter manufacture.Digital microfluidicshas been described as a means for carrying outDigital PCR.

Molecular biology

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In addition to microarrays, biochips have been designed for two-dimensionalelectrophoresis,[87]transcriptomeanalysis,[88]andPCRamplification.[89]Other applications include various electrophoresis andliquid chromatographyapplications for proteins andDNA,cell separation, in particular, blood cell separation, protein analysis, cell manipulation and analysis including cell viability analysis[34]andmicroorganismcapturing.[74]

Evolutionary biology

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By combining microfluidics withlandscape ecologyandnanofluidics,a nano/micro fabricated fluidic landscape can be constructed by building local patches ofbacterialhabitatand connecting them by dispersal corridors. The resulting landscapes can be used as physical implementations of anadaptive landscape,[90]by generating a spatial mosaic of patches of opportunity distributed in space and time. The patchy nature of these fluidic landscapes allows for the study of adapting bacterial cells in ametapopulationsystem. Theevolutionary ecologyof these bacterial systems in thesesynthetic ecosystemsallows for usingbiophysicsto address questions inevolutionary biology.

Cell behavior

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Main article:Microfluidic cell culture

The ability to create precise and carefully controlledchemoattractantgradients makes microfluidics the ideal tool to study motility,[91]chemotaxisand the ability to evolve / develop resistance to antibiotics in small populations of microorganisms and in a short period of time. These microorganisms includingbacteria[92]and the broad range of organisms that form the marinemicrobial loop,[93]responsible for regulating much of the oceans' biogeochemistry.

Microfluidics has also greatly aided the study ofdurotaxisby facilitating the creation of durotactic (stiffness) gradients.

Cellular biophysics

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By rectifying the motion of individual swimming bacteria,[94]microfluidic structures can be used to extract mechanical motion from a population of motile bacterial cells.[95]This way, bacteria-powered rotors can be built.[96][97]

Optics

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The merger of microfluidics and optics is typical known asoptofluidics.Examples of optofluidic devices are tunable microlens arrays[98][99]and optofluidic microscopes.

Microfluidic flow enables fast sample throughput, automated imaging of large sample populations, as well as 3D capabilities,[100][101]or superresolution.[102]

Photonics Lab on a Chip (PhLOC)

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Due to the increase in safety concerns and operating costs of common analytic methods (ICP-MS,ICP-AAS,andICP-OES[103]), the Photonics Lab on a Chip (PhLOC) is becoming an increasingly popular tool for the analysis of actinides and nitrates in spent nuclear waste. The PhLOC is based on the simultaneous application ofRamanandUV-Vis-NIRspectroscopy,[104]which allows for the analysis of more complex mixtures which contain several actinides at different oxidation states.[105]Measurements made with these methods have been validated at the bulk level for industrial tests,[103][106]and are observed to have a much lower variance at the micro-scale.[107]This approach has been found to have molar extinction coefficients (UV-Vis) in line with known literature values over a comparatively large concentration span for 150 μL[105]via elongation of the measurement channel, and obeysBeer's Lawat the micro-scale for U(IV).[108]Through the development of a spectrophotometric approach to analyzing spent fuel, an on-line method for measurement of reactant quantities is created, increasing the rate at which samples can be analyzed and thus decreasing the size of deviations detectable within reprocessing.[106]

Through the application of the PhLOC, flexibility and safety of operational methods are increased. Since the analysis of spent nuclear fuel involves extremely harsh conditions, the application of disposable and rapidly produced devices (Based on castable and/or engravable materials such as PDMS, PMMA, and glass[109]) is advantageous, although material integrity must be considered under specific harsh conditions.[108]Through the usage of fiber optic coupling, the device can be isolated from instrumentation, preventing irradiative damage and minimizing the exposure of lab personnel to potentially harmful radiation, something not possible on the lab scale nor with the previous standard of analysis.[105]The shrinkage of the device also allows for lower amounts of analyte to be used, decreasing the amount of waste generated and exposure to hazardous materials.[105]

Expansion of the PhLOC to miniaturize research of the full nuclear fuel cycle is currently being evaluated, with steps of thePUREXprocess successfully being demonstrated at the micro-scale.[104]Likewise, the microfluidic technology developed for the analysis of spent nuclear fuel is predicted to expand horizontally to analysis of other actinide, lanthanides, and transition metals with little to no modification.[105]

High Performance Liquid Chromatography (HPLC)

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HPLC in the field of microfluidics comes in two different forms. Early designs included running liquid through the HPLC column then transferring the eluted liquid to microfluidic chips and attaching HPLC columns to the microfluidic chip directly.[110]The early methods had the advantage of easier detection from certain machines like those that measure fluorescence.[111]More recent designs have fully integrated HPLC columns into microfluidic chips. The main advantage of integrating HPLC columns into microfluidic devices is the smaller form factor that can be achieved, which allows for additional features to be combined within one microfluidic chip. Integrated chips can also be fabricated from multiple different materials, including glass and polyimide which are quite different from the standard material ofPDMSused in many different droplet-based microfluidic devices.[112][113]This is an important feature because different applications of HPLC microfluidic chips may call for different pressures. PDMS fails in comparison for high-pressure uses compared to glass and polyimide. High versatility of HPLC integration ensures robustness by avoiding connections and fittings between the column and chip.[114]The ability to build off said designs in the future allows the field of microfluidics to continue expanding its potential applications.

The potential applications surrounding integrated HPLC columns within microfluidic devices have proven expansive over the last 10–15 years. The integration of such columns allows for experiments to be run where materials were in low availability or very expensive, like in biological analysis of proteins. This reduction in reagent volumes allows for new experiments like single-cell protein analysis, which due to size limitations of prior devices, previously came with great difficulty.[115]The coupling of HPLC-chip devices with other spectrometry methods like mass-spectrometry allow for enhanced confidence in identification of desired species, like proteins.[116]Microfluidic chips have also been created with internal delay-lines that allow for gradient generation to further improve HPLC, which can reduce the need for further separations.[117]Some other practical applications of integrated HPLC chips include the determination of drug presence in a person through their hair[118]and the labeling of peptides through reverse phase liquid chromatography.[119]

Acoustic droplet ejection (ADE)

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Acoustic droplet ejectionuses a pulse ofultrasoundto move low volumes offluids(typically nanoliters or picoliters) without any physical contact. This technology focuses acoustic energy into a fluid sample to eject droplets as small as a millionth of a millionth of a litre (picoliter = 10−12litre). ADE technology is a very gentle process, and it can be used to transfer proteins, high molecular weight DNA and live cells without damage or loss of viability. This feature makes the technology suitable for a wide variety of applications includingproteomicsand cell-based assays.

Fuel cells

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Further information:Electroosmotic pump

Microfluidicfuel cellscan use laminar flow to separate the fuel and its oxidant to control the interaction of the two fluids without the physical barrier that conventional fuel cells require.[120][121][122]

Astrobiology

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To understand the prospects for life to exist elsewhere in the universe,astrobiologistsare interested in measuring the chemical composition of extraplanetary bodies.[123]Because of their small size and wide-ranging functionality, microfluidic devices are uniquely suited for these remote sample analyses.[124][125][126]From an extraterrestrial sample, the organic content can be assessed using microchipcapillary electrophoresisand selective fluorescent dyes.[127]These devices are capable of detectingamino acids,[128]peptides,[129]fatty acids,[130]and simplealdehydes,ketones,[131]andthiols.[132]These analyses coupled together could allow powerful detection of the key components of life, and hopefully inform our search for functioning extraterrestrial life.[133]

Food science

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Microfluidic techniques such as droplet microfluidics, paper microfluidics, andlab-on-a-chipare used in the realm of food science in a variety of categories.[134]Research in nutrition,[135][136]food processing, and food safety benefit from microfluidic technique because experiments can be done with less reagents.[134]

Food processing requires the ability to enable shelf stability in foods, such as emulsions or additions of preservatives. Techniques such as droplet microfluidics are used to create emulsions that are more controlled and complex than those created by traditional homogenization due to the precision of droplets that is achievable. Using microfluidics for emulsions is also more energy efficient compared to homogenization in which “only 5% of the supplied energy is used to generate the emulsion, with the rest dissipated as heat”.[137]Although these methods have benefits, they currently lack the ability to be produced at large scale that is needed for commercialization.[138]Microfluidics are also used in research as they allow for innovation in food chemistry and food processing.[134][138]An example in food engineering research is a novel micro-3D-printed device fabricated to research production of droplets for potential food processing industry use, particularly in work with enhancing emulsions.[139]

Paper and droplet microfluidics allow for devices that can detect small amounts of unwanted bacteria or chemicals, making them useful in food safety and analysis.[140]Paper-based microfluidic devices are often referred to as microfluidic paper-based analytical devices (μPADs) and can detect such things as nitrate,[141]preservatives,[142]or antibiotics[143]in meat by a colorimetric reaction that can be detected with a smartphone. These methods are being researched because they use less reactants, space, and time compared to traditional techniques such as liquid chromatography. μPADs also make home detection tests possible, which is of interest to those with allergies and intolerances.[141]In addition to paper-based methods, research demonstrates droplet-based microfluidics shows promise in drastically shortening the time necessary to confirm viable bacterial contamination in agricultural waters in the domestic and international food industry.[140]

Future directions

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Microfluidics for personalized cancer treatment

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Personalized cancer treatment is a tuned method based on the patient's diagnosis and background. Microfluidic technology offers sensitive detection with higher throughput, as well as reduced time and costs. For personalized cancer treatment, tumor composition and drug sensitivities are very important.[144]

A patient's drug response can be predicted based on the status ofbiomarkers,or the severity and progression of the disease can be predicted based on the atypical presence of specific cells.[145]Drop-qPCRis adroplet microfluidictechnology in which droplets are transported in a reusable capillary and alternately flow through two areas maintained at different constant temperatures and fluorescence detection. It can be efficient with a low contamination risk to detectHer2.[144]Adigitaldroplet‐basedPCRmethod can be used to detect theKRASmutations withTaqMan probes,to enhance detection of the mutative gene ratio.[146]In addition, accurate prediction of postoperative disease progression inbreastorprostate cancerpatients is essential for determining post-surgery treatment. A simple microfluidic chamber, coated with a carefully formulated extracellular matrix mixture is used for cells obtained from tumorbiopsyafter 72 hours of growth and a thorough evaluation of cells by imaging.[147]

Microfluidics is also suitable forcirculating tumor cells (CTCs)and non-CTCsliquid biopsyanalysis. Beads conjugate to anti‐epithelial cell adhesion molecule (EpCAM)antibodies forpositive selectionin theCTCsisolation chip (iCHIP).[148]CTCscan also be detected by using the acidification of thetumor microenvironmentand the difference in membrane capacitance.[149][150]CTCsare isolated from blood by a microfluidic device, and are culturedon-chip,which can be a method to capture more biological information in a single analysis. For example, it can be used to test the cell survival rate of 40 different drugs or drug combinations.[151]Tumor‐derivedextracellular vesiclescan be isolated from urine and detected by an integrated double‐filtration microfluidic device; they also can be isolated from blood and detected byelectrochemical sensing methodwith a two‐level amplificationenzymatic assay.[152][153]

Tumor materials can directly be used for detection through microfluidic devices. To screenprimary cellsfor drugs, it is often necessary to distinguish cancerous cells from non-cancerous cells. Amicrofluidic chipbased on the capacity of cells to pass small constrictions can sort the cell types,metastases.[154]Droplet‐based microfluidicdevices have the potential to screen different drugs or combinations of drugs, directly on theprimary tumorsample with high accuracy. To improve this strategy, the microfluidic program with a sequential manner of drug cocktails, coupled with fluorescent barcodes, is more efficient.[155]Another advanced strategy is detecting growth rates of single-cell by using suspended microchannel resonators, which can predict drug sensitivities of rareCTCs.[156]

Microfluidics devices also can simulate thetumor microenvironment,to help to test anticancer drugs. Microfluidic devices with 2D or3D cell culturescan be used to analyze spheroids for different cancer systems (such aslung cancerandovarian cancer), and are essential for multiple anti-cancer drugs and toxicity tests. This strategy can be improved by increasing the throughput and production of spheroids. For example, onedroplet-based microfluidicdevice for3D cell cultureproduces 500 spheroids per chip.[157]These spheroids can be cultured longer in different surroundings to analyze and monitor. The other advanced technology isorgans‐on‐a‐chip,and it can be used to simulate several organs to determine the drug metabolism and activity based onvesselsmimicking, as well as mimicpH,oxygen... to analyze the relationship between drugs and human organ surroundings.[157]

A recent strategy is single-cellchromatin immunoprecipitation (ChiP)‐Sequencingindroplets,which operates by combining droplet‐based single cellRNA sequencingwithDNA‐barcodedantibodies, possibly to explore thetumor heterogeneityby thegenotypeandphenotypeto select the personalized anti-cancer drugs and prevent the cancer relapse.[158]

Advancements in Capillary Electrophoresis (CE) Systems

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One significant advancement in the field is the development of integratedcapillary electrophoresis(CE) systems onmicrochips,as demonstrated byZ. Hugh Fanand D. Jed. Harrison. They created a planar glass chip incorporating a sample injector and separation channels usingmicromachiningtechniques. This setup allowed for the rapid separation ofamino acidsin just a few seconds, achieving high separation efficiencies with up to 6800theoretical plates.The use of highelectric fields,possible due to thethermal massandconductivityof glass, minimized Joule heating effects, making the system highly efficient and fast. Such innovations highlight the potential of microfluidic devices in analytical chemistry, particularly in applications requiring quick and precise analyses.[159]

See also

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References

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