TANGO2
Transport and golgi organization 2 homolog(TANGO2) also known aschromosome 22open reading frame25(C22orf25) is aproteinthat in humans is encoded by the TANGO2 gene.
The function of C22orf25 is not currently known. It is characterized by the NRDE superfamily domain (DUF883), which is strictly known for the conservedamino acidsequence of (N)-Asparagine(R)-Arginine(D)-Aspartic Acid(E)-Glutamic Acid.This domain is found among distantly relatedspeciesfrom the six kingdoms:[4]Eubacteria,Archaebacteria,Protista,Fungi,Plantae,andAnimaliaand is known to be involved inGolgiorganization and protein secretion.[5]It is likely that it localizes in thecytoplasmbut is anchored in thecell membraneby the second amino acid.[6][7]C22orf25 is alsoxenologousto T10 like proteins in theFowlpox VirusandCanarypox Virus.The gene coding for C22orf25 is located onchromosome 22and the location q11.21, so it is often associated with22q11.2 deletion syndrome.[8]
Protein
[edit]Gene Size | Protein Size | # of exons | Promoter Sequence | Signal Peptide | Molecular Weight | Domain Length |
---|---|---|---|---|---|---|
2271 bp | 276 aa | 9[9] | 687 bp | No[10] | 30.9 kDa[11] | 270 aa |
Gene neighborhood
[edit]The C22orf25 gene is located on the long arm (q) of chromosome 22 in region 1, band 1, and sub-band 2 (22q11.21) starting at 20,008,631base pairsand ending at 20,053,447 base pairs.[8]There is a 1.5-3.0 Mb deletion containing around 30-40genes,spanning this region that causes the most survivable genetic deletion disorder known as22q11.2 deletion syndrome,which is most commonly known as DiGeorge syndrome or Velocaridofacial syndrome.[12][13]22q11.2 deletion syndrome has a vast array ofphenotypesand is not attributed to the loss of a single gene. The vast phenotypes arise from deletions of not only DiGeorge Syndrome Critical Region (DGCR) genes and disease genes but other unidentified genes as well.[14]
C22orf25 is in close proximity to DGCR8 as well as other genes known to play a part in DiGeorge Syndrome such as armadillo repeat gene deleted in Velocardiofacial syndrome (ARVCF), Cathechol-O-methyltransferase (COMT) and T-box 1 (TBX1).[15][16]
Predicted mRNA features
[edit]Promoter
[edit]Thepromoterfor the C22orf25 gene spans 687 base pairs from 20,008,092 to 20,008,878 with a predictedtranscriptional start sitethat is 104 base pairs and spans from 20,008,591 to 20,008,694.[17]The promoter region and beginning of the C22orf25 gene (20,008,263 to 20,009,250) is not conserved past primates. This region was used to determinetranscription factorinteractions.
Transcription factors
[edit]Some of the main transcription factors that bind to the promoter are listed below.[18]
Reference | Detailed Family Information | Start (amino acid) | End (amino acid) | Strand |
---|---|---|---|---|
XBBF | X-box binding factors | 227 | 245 | - |
GCMF | Chorion-specific transcription factors (with a GCM DNA binding domain) | 151 | 165 | - |
YBXF | Y-box binding transcription factors | 158 | 170 | - |
RUSH | SWI/SNF related nucleophosphoproteins (with a RING finger binding motif) | 222 | 232 | - |
NEUR | NeuroD, Beta2, HLH domain | 214 | 226 | - |
PCBE | PREB core-binding element | 148 | 162 | - |
NR2F | Nuclear receptor subfamily 2 factors | 169 | 193 | - |
AP1R | MAF and AP1 related factors | 201 | 221 | - |
ZF02 | C2H2 zinc finger transcription factors 2 | 108 | 130 | - |
TALE | TALE homeodomain class recognizing TG motifs | 216 | 232 | - |
WHNF | Winged helix transcription factors | 271 | 281 | - |
FKHD | Forkhead domain factors | 119 | 135 | + |
MYOD | Myoblast determining factors | 218 | 234 | + |
AP1F | AP1, activating protein 1 | 118 | 130 | + |
BCL6 | POZ domain zinc finger expressed in B cells | 190 | 206 | + |
CARE | Calcium response elements | 196 | 206 | + |
EVI1 | EVI1 nuclear transcription factor | 90 | 106 | + |
ETSF | ETS transcription factor | 162 | 182 | + |
TEAF | TEA/ATTS DNA binding domain factors | 176 | 188 | + |
Expression analysis
[edit]Expression data fromExpressed Sequence Tagmapping,microarrayandin situhybridizationshow high expression forHomo sapiensin theblood,bone marrowandnerves.[19][20][21]Expression is not restricted to these areas and low expression is seen elsewhere in the body. InCaenorhabditis elegans,the snt-1 gene (C22orf25 homologue) was expressed in the nerve ring, ventral and dorsal cord processes, sites of neuromuscular junctions, and in neurons.[22]
Evolutionary history
[edit]The NRDE (DUF883) domain, is a domain of unknown function spanning majority of the C22orf25 gene and is found among distantly related species, including viruses.
Genus and Species | Common Name | Accession Number | Seq. Length |
Seq. Identity |
Seq. Similarity |
Kingdom | Time of Divergence |
---|---|---|---|---|---|---|---|
Homo sapiens | humans | NP_690870.3 | 276aa | - | - | Animalia | - |
Pan troglodytes | common chimpanzee | BAK62258.1 | 276aa | 99% | 100% | Animalia | 6.4 mya |
Ailuropoda melanoleuca | giant panda | XP_002920626 | 276aa | 91% | 94% | Animalia | 94.4 mya |
Mus musculus | house mouse | NP_613049.2 | 276aa | 88% | 95% | Animalia | 92.4 mya |
Meleagris gallopavo | turkey | XP_003210928 | 276aa | 74% | 88% | Animalia | 301.7 mya |
Gallus gallus | Red Junglefowl | NP_001007837 | 276aa | 73% | 88% | Animalia | 301.7 mya |
Xenopus laevis | African clawed frog | NP_001083694 | 275aa | 69% | 86% | Animalia | 371.2 mya |
Xenopus (Silurana) tropicalis | Western clawed frog | NP_001004885.1 | 276aa | 68% | 85% | Animalia | 371.2 mya |
Salmo salar | Atlantic salmon | NP_001167100 | 274aa | 66% | 79% | Animalia | 400.1 mya |
Danio rerio | zebrafish | NP_001003781 | 273aa | 64% | 78% | Animalia | 400.1 mya |
Canarypox | virus | NP_955117 | 275aa | 50% | 69% | - | - |
Fowlpox | virus | NP_039033 | 273aa | 44% | 63% | - | - |
Cupriavidus | proteobacteria | YP_002005507.1 | 275aa | 38% | 52% | Eubacteria | 2313.2 mya |
Burkholderia | proteobacteria | YP_004977059 | 273aa | 37% | 53% | Eubacteria | 2313.2 mya |
Physcomitrella patens | moss | XP_001781807 | 275aa | 37% | 54% | Plantae | 1369 mya |
Zea mays | maize/corn | ACG35095 | 266aa | 33% | 53% | Plantae | 1369 mya |
Trichophyton rubrum | fungus | XP_003236126 | 306aa | 32% | 47% | Fungi | 1215.8 mya |
Sporisorium reilianum | Plant pathogen | CBQ69093 | 321aa | 32% | 43% | Fungi | 1215.8 mya |
Perkinsus marinus | pathogen of oysters | XP_002787624 | 219aa | 31% | 48% | Protista | 1381.2 mya |
Tetrahymena thermophilia | Ciliate protozoa | XP_001010229 | 277aa | 26% | 44% | Protista | 1381.2 mya |
Natrialba magadii | extremophile | YP_003481665 | 300aa | 25% | 39% | Archaebacteria | 3556.3 mya |
Halopiger xanaduensis | halophilic archaeon | YP_004597780.1 | 264aa | 24% | 39% | Archaebacteria | 3556.3 mya |
Predicted protein features
[edit]Post translational modifications
[edit]Post translational modifications of the C22orf25 gene that are evolutionarily conserved in the Animalia and Plantae kingdoms as well as the Canarypox Virus includeglycosylation(C-mannosylation),[23]glycation,[24]phosphorylation(kinase specific),[25]andpalmitoylation.[26]
Predicted topology
[edit]C22orf25 localizes to the cytoplasm and is anchored to the cell membrane by the second amino acid. As mentioned previously, the second amino acid is modified by palmitoylation. Palmitoylation is known to contribute to membrane association[27]because it contributes to enhanced hydrophobicity.[6]Palmitoylation is known to play a role in the modulation of proteins' trafficking,[28]stability[29]and sorting.[30]Palmitoylation is also involved in cellular signaling[31]and neuronal transmission.[32]
Protein Interactions
[edit]C22orf25 has been shown to interact withNFKB1,[33]RELA,[33]RELB,[33]BTRC,[33]RPS27A,[33]BCL3,[33]MAP3K8,[33]NFKBIA,[33]SIN3A,[33]SUMO1,[33]Tat.[34]
Clinical significance
[edit]Mutations in the TANGO2 gene may cause defects in mitochondrialβ-oxidation[35]and increasedendoplasmic reticulumstress and a reduction inGolgivolume density.[36]These mutations results in early onsethypoglycemia,hyperammonemia,rhabdomyolysis,cardiac arrhythmias,andencephalopathythat later develops into cognitive impairment.[35][36]
References
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- ^"Human PubMed Reference:".National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^"Mouse PubMed Reference:".National Center for Biotechnology Information, U.S. National Library of Medicine.
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- ^"Statistical Analysis of Protein Sequence (Biology Workbench)".[permanent dead link]
- ^Meechan DW, Maynard TM, Tucker ES, LaMantia AS (2011)."Three phases of DiGeorge/22q11 deletion syndrome pathogenesis during brain development: Patterning, proliferation, and mitochondrial functions of 22q11 genes".International Journal of Developmental Neuroscience.29(3): 283–294.doi:10.1016/j.ijdevneu.2010.08.005.PMC3770287.PMID20833244.
- ^Kniffin C."DiGeorge Syndrome; DGS. Retrieved April 2012, from Online Mendelian Inheritance in Man".
- ^Scambler PJ (2000)."The 22q11 deletion syndromes".Hum. Mol. Genet.9(16): 2421–6.doi:10.1093/hmg/9.16.2421.PMID11005797.
- ^Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH, Gripp KW, Amemiya A, McDonald-Mcginn DM, Hain HS, Emanuel BS, Zackai EH (1993)."22q11.2 Deletion Syndrome".University of Washington, Seattle.PMID20301696.
- ^"BLAT UCSC Genome Browser".
- ^"El Durado (Genomatix)".
- ^"El Durado-Genomatix".
- ^"Unigene NCBI".Archived fromthe originalon 2013-07-12.Retrieved2012-04-26.
- ^"GEO Profiles NCBI".
- ^"Bio GPS".
- ^"WormBase".
- ^"NetCGly (ExPASy)".Archived fromthe originalon 2012-04-24.Retrieved2012-04-28.
- ^"NetGlycate (ExPASy)".Archived fromthe originalon 2012-04-24.Retrieved2012-04-28.
- ^"Phos (ExPASy)".Archived fromthe originalon 2012-04-24.Retrieved2012-04-28.
- ^"CSS Palm (ExPASy)".Archived fromthe originalon 2012-04-24.Retrieved2012-04-28.
- ^Resh MD (2006). "Palmitoylation of Ligands, Receptors, and Intracellular Signaling Molecules".Science's STKE.2006(359): 14.doi:10.1126/stke.3592006re14.PMID17077383.S2CID25729573.
- ^Draper JM, Xia Z, Smith CD (Aug 2007)."Cellular palmitoylation and trafficking of lipated peptides".Journal of Lipid Research.48(8): 1873–1884.doi:10.1194/jlr.m700179-jlr200.PMC2895159.PMID17525474.
- ^Linder ME, Deschenes RJ (Jan 2007). "Palmitoylation: policing protein stability and traffic".Nature Reviews Molecular Cell Biology.8(1): 74–84.doi:10.1038/nrm2084.PMID17183362.S2CID26339042.
- ^Greaves J, Chamberlain LH (Jan 2007)."Palmitoylation-dependent protein sorting".The Journal of Cell Biology.176(3): 249–254.doi:10.1083/jcb.200610151.PMC2063950.PMID17242068.
- ^Casey PJ (1995). "Protein lipidation in cell signaling".Science.268(5208): 221–5.Bibcode:1995Sci...268..221C.doi:10.1126/science.7716512.PMID7716512.
- ^Roth AF, Wan J, Bailey AO, Sun B, Kuchar JA, Green WN, Phinney BS, Yates JR, Davis NG (June 2006)."Global analysis of protein palmitoylation in yeast".Cell.125(5): 1003–1013.doi:10.1016/j.cell.2006.03.042.PMC2246083.PMID16751107.
- ^abcdefghij"Molecular Interaction Database".Archived fromthe originalon 2006-05-06.
- ^"Viral Molecular Interaction Database".Archived fromthe originalon 2015-02-15.
- ^abKremer LS, Distelmaier F, Alhaddad B, Hempel M, Iuso A, Küpper C, et al. (2016)."Bi-allelic Truncating Mutations in TANGO2 Cause Infancy-Onset Recurrent Metabolic Crises with Encephalocardiomyopathy".American Journal of Human Genetics.98(2): 358–62.doi:10.1016/j.ajhg.2015.12.009.PMC4746337.PMID26805782.
- ^abLalani SR, Liu P, Rosenfeld JA, Watkin LB, Chiang T, Leduc MS, et al. (2016)."Recurrent Muscle Weakness with Rhabdomyolysis, Metabolic Crises, and Cardiac Arrhythmia Due to Bi-allelic TANGO2 Mutations".American Journal of Human Genetics.98(2): 347–57.doi:10.1016/j.ajhg.2015.12.008.PMC4746334.PMID26805781.
External links
[edit]- [1]Archived2017-11-07 at theWayback Machinewww.tango2.it - Disease website
- [2]www.tango2research.org - Research disease website -