Furin
Furinis aprotease,a proteolyticenzymeactivated bysubstrate presentationthat in humans and other animals is encoded by theFURINgene.Some proteins are inactive when they are first synthesized, and must have sections removed in order to become active. Furin cleaves these sections and activates the proteins.[5][6][7][8]It was named furin because it was in the upstream region of anoncogeneknown asFES.The gene was known as FUR (FES Upstream Region) and therefore the protein was named furin. Furin is also known asPACE(Paired basicAmino acidCleavingEnzyme). A member offamily S8,furin is asubtilisin-like peptidase.
Function
[edit]Theproteinencoded by this gene is anenzymethat belongs to thesubtilisin-likeproprotein convertasefamily. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serineendoproteasethat can efficiently cleave precursor proteins at their paired basic amino acid processing sites. Some of its substrates are: proparathyroid hormone,transforming growth factor beta 1precursor, proalbumin,pro-beta-secretase,membrane type-1 matrix metalloproteinase,beta subunit of pro-nerve growth factorandvon Willebrand factor.A furin-like pro-protein convertase has been implicated in the processing of RGMc (also calledhemojuvelin), a gene involved in a severe iron-overload disorder called juvenile hemochromatosis. Both the Ganz and Rotwein groups demonstrated that furin-likeproprotein convertases(PPC) are responsible for conversion of 50 kDa HJV to a 40 kDa protein with a truncated COOH-terminus, at a conserved polybasic RNRR site. This suggests a potential mechanism to generate the soluble forms of HJV/hemojuvelin (s-hemojuvelin) found in the blood of rodents and humans.[9][10]
The furin substrates and the locations of furin cleavage sites in protein sequences can be predicted by two bioinformatics methods: ProP[11]and PiTou.[12]
Clinical significance
[edit]Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursorgp160togp120andgp41prior to viral assembly.[13]This protease is also thought to play a role in tumor progression.[7]The use of alternate polyadenylation sites has been found for the FURIN gene.[citation needed]
Furin is enriched in theGolgi apparatus,where it functions to cleave other proteins into their mature/active forms.[14]Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg'). In addition to processing cellular precursor proteins, furin is also used by a number of pathogens. For example, the envelope proteins of viruses such asHIV,influenza,dengue fever,several filoviruses includingebolaandmarburg virus,and the spike protein ofSARS-CoV-2,[15][16][17]must be cleaved by furin or furin-like proteases to become fully functional. When SARS-CoV-2 virus is being synthesized in an infected cell, furin or furin-like proteases cleave thespike proteininto two portions (S1 and S2), which remain associated.[18]
Anthrax toxin,Pseudomonasexotoxin, andpapillomavirusesmust be processed by furin during their initial entry into host cells. Inhibitors of furin are under consideration as therapeutic agents for treatinganthraxinfection.[19]
Furin is regulated by cholesterol andsubstrate presentation.When cholesterol is high, furin traffics toGM1lipid rafts.When cholesterol is low, furin traffics to the disordered region.[20]This is speculated to contribute to cholesterol and age dependent priming of SARS-CoV.
Expression of furin in T-cells is required for maintenance ofperipheral immune tolerance.[21]
Interactions
[edit]Furin has been shown tointeractwithPACS1.[22]
References
[edit]- ^abcGRCh38: Ensembl release 89: ENSG00000140564–Ensembl,May 2017
- ^abcGRCm38: Ensembl release 89: ENSMUSG00000030530–Ensembl,May 2017
- ^"Human PubMed Reference:".National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^"Mouse PubMed Reference:".National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^Wise RJ, Barr PJ, Wong PA, Kiefer MC, Brake AJ, Kaufman RJ (December 1990)."Expression of a human proprotein processing enzyme: correct cleavage of the von Willebrand factor precursor at a paired basic amino acid site".Proceedings of the National Academy of Sciences of the United States of America.87(23): 9378–82.Bibcode:1990PNAS...87.9378W.doi:10.1073/pnas.87.23.9378.PMC55168.PMID2251280.
- ^Kiefer MC, Tucker JE, Joh R, Landsberg KE, Saltman D, Barr PJ (December 1991). "Identification of a second human subtilisin-like protease gene in the fes/fps region of chromosome 15".DNA and Cell Biology.10(10): 757–69.doi:10.1089/dna.1991.10.757.PMID1741956.
- ^ab"Entrez Gene: FURIN furin (paired basic amino acid cleaving enzyme)".
- ^Roebroek AJ, Schalken JA, Leunissen JA, Onnekink C, Bloemers HP, Van de Ven WJ (September 1986)."Evolutionary conserved close linkage of the c-fes/fps proto-oncogene and genetic sequences encoding a receptor-like protein".The EMBO Journal.5(9): 2197–202.doi:10.1002/j.1460-2075.1986.tb04484.x.PMC1167100.PMID3023061.
- ^Lin L, Nemeth E, Goodnough JB, Thapa DR, Gabayan V, Ganz T (2008)."Soluble hemojuvelin is released by proprotein convertase-mediated cleavage at a conserved polybasic RNRR site".Blood Cells, Molecules & Diseases.40(1): 122–31.doi:10.1016/j.bcmd.2007.06.023.PMC2211380.PMID17869549.
- ^Kuninger D, Kuns-Hashimoto R, Nili M, Rotwein P (April 2008)."Pro-protein convertases control the maturation and processing of the iron-regulatory protein, RGMc/hemojuvelin".BMC Biochemistry.9:9.doi:10.1186/1471-2091-9-9.PMC2323002.PMID18384687.
- ^Duckert P, Brunak S, Blom N (January 2004)."Prediction of proprotein convertase cleavage sites".Protein Engineering, Design & Selection.17(1): 107–12.doi:10.1093/protein/gzh013.PMID14985543.
- ^Tian S, Huajun W, Wu J (February 2012)."Computational prediction of furin cleavage sites by a hybrid method and understanding mechanism underlying diseases".Scientific Reports.2:261.Bibcode:2012NatSR...2E.261T.doi:10.1038/srep00261.PMC3281273.PMID22355773.
- ^Hallenberger S, Bosch V, Angliker H, Shaw E, Klenk HD, Garten W (November 1992). "Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gp160".Nature.360(6402): 358–61.Bibcode:1992Natur.360..358H.doi:10.1038/360358a0.PMID1360148.S2CID4306605.
- ^Thomas G (October 2002)."Furin at the cutting edge: from protein traffic to embryogenesis and disease".Nature Reviews. Molecular Cell Biology.3(10): 753–66.doi:10.1038/nrm934.PMC1964754.PMID12360192.
- ^Coutard B, Valle C, de Lamballerie X, Canard B, Seidah NG, Decroly E (April 2020)."The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade".Antiviral Research.176:104742.doi:10.1016/j.antiviral.2020.104742.PMC7114094.PMID32057769.
- ^Hoffmann M, Kleine-Weber H, Pöhlmann S (May 2020)."A Multibasic Cleavage Site in the Spike Protein of SARS-CoV-2 Is Essential for Infection of Human Lung Cells".Molecular Cell.78(4): 779–784.e5.doi:10.1016/j.molcel.2020.04.022.PMC7194065.PMID32362314.
- ^"The origin of COVID-19: Evidence piles up, but the jury's still out".11 October 2021.
The furin cleavage site on the SARS-CoV-2 virus allows its spikes to be cut and "primed" as it moves out of one cell and into another. The site is thought to make the virus more transmissible.
- ^Jackson CB, Farzan M, Chen B, Choe H (2022)."Mechanisms of SARS-CoV-2 entry into cells".Nature Reviews Molecular Cell Biology.23(1): 3–20.doi:10.1038/s41580-021-00418-x.PMC8491763.PMID34611326.
- ^Shiryaev SA, Remacle AG, Ratnikov BI, Nelson NA, Savinov AY, Wei G, et al. (July 2007)."Targeting host cell furin proprotein convertases as a therapeutic strategy against bacterial toxins and viral pathogens".The Journal of Biological Chemistry.282(29): 20847–53.doi:10.1074/jbc.M703847200.PMID17537721.
- ^Wang H, Yuan Z, Pavel MA, Jablonski SM, Jablonski J, Hobson R, et al. (June 2021)."The role of high cholesterol in age-related COVID19 lethality".bioRxiv:2020.05.09.086249.doi:10.1101/2020.05.09.086249.PMC7263494.PMID32511366.
- ^Pesu M, Watford WT, Wei L, Xu L, Fuss I, Strober W, et al. (September 2008)."T-cell-expressed proprotein convertase furin is essential for maintenance of peripheral immune tolerance".Nature.455(7210): 246–50.Bibcode:2008Natur.455..246P.doi:10.1038/nature07210.PMC2758057.PMID18701887.
- ^Wan L, Molloy SS, Thomas L, Liu G, Xiang Y, Rybak SL, Thomas G (July 1998)."PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization".Cell.94(2): 205–16.doi:10.1016/S0092-8674(00)81420-8.PMID9695949.S2CID15027198.
Further reading
[edit]- Nakayama K (November 1997)."Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins".The Biochemical Journal.327 ( Pt 3) (3): 625–35.doi:10.1042/bj3270625.PMC1218878.PMID9599222.
- Bassi DE, Mahloogi H, Klein-Szanto AJ (June 2000). "The proprotein convertases furin and PACE4 play a significant role in tumor progression".Molecular Carcinogenesis.28(2): 63–9.doi:10.1002/1098-2744(200006)28:2<63::AID-MC1>3.0.CO;2-C.PMID10900462.S2CID22849623.
- Hallenberger S, Bosch V, Angliker H, Shaw E, Klenk HD, Garten W (November 1992). "Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gp160".Nature.360(6402): 358–61.Bibcode:1992Natur.360..358H.doi:10.1038/360358a0.PMID1360148.S2CID4306605.
- Rehemtulla A, Kaufman RJ (May 1992)."Preferred sequence requirements for cleavage of pro-von Willebrand factor by propeptide-processing enzymes".Blood.79(9): 2349–55.doi:10.1182/blood.V79.9.2349.2349.PMID1571548.
- Leduc R, Molloy SS, Thorne BA, Thomas G (July 1992)."Activation of human furin precursor processing endoprotease occurs by an intramolecular autoproteolytic cleavage".The Journal of Biological Chemistry.267(20): 14304–8.doi:10.1016/S0021-9258(19)49712-3.PMID1629222.
- Barr PJ, Mason OB, Landsberg KE, Wong PA, Kiefer MC, Brake AJ (June 1991). "cDNA and gene structure for a human subtilisin-like protease with cleavage specificity for paired basic amino acid residues".DNA and Cell Biology.10(5): 319–28.doi:10.1089/dna.1991.10.319.PMID1713771.
- Herz J, Kowal RC, Goldstein JL, Brown MS (June 1990)."Proteolytic processing of the 600 kd low density lipoprotein receptor-related protein (LRP) occurs in a trans-Golgi compartment".The EMBO Journal.9(6): 1769–76.doi:10.1002/j.1460-2075.1990.tb08301.x.PMC551881.PMID2112085.
- van den Ouweland AM, van Duijnhoven HL, Keizer GD, Dorssers LC, Van de Ven WJ (February 1990)."Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2".Nucleic Acids Research.18(3): 664.doi:10.1093/nar/18.3.664.PMC333486.PMID2408021.
- Van den Ouweland AM, Van Groningen JJ, Roebroek AJ, Onnekink C, Van de Ven WJ (September 1989)."Nucleotide sequence analysis of the human fur gene".Nucleic Acids Research.17(17): 7101–2.doi:10.1093/nar/17.17.7101.PMC318436.PMID2674906.
- Roebroek AJ, Schalken JA, Leunissen JA, Onnekink C, Bloemers HP, Van de Ven WJ (September 1986)."Evolutionary conserved close linkage of the c-fes/fps proto-oncogene and genetic sequences encoding a receptor-like protein".The EMBO Journal.5(9): 2197–202.doi:10.1002/j.1460-2075.1986.tb04484.x.PMC1167100.PMID3023061.
- Molloy SS, Thomas L, VanSlyke JK, Stenberg PE, Thomas G (January 1994)."Intracellular trafficking and activation of the furin proprotein convertase: localization to the TGN and recycling from the cell surface".The EMBO Journal.13(1): 18–33.doi:10.1002/j.1460-2075.1994.tb06231.x.PMC394775.PMID7508380.
- Brakch N, Dettin M, Scarinci C, Seidah NG, Di Bello C (August 1995). "Structural investigation and kinetic characterization of potential cleavage sites of HIV GP160 by human furin and PC1".Biochemical and Biophysical Research Communications.213(1): 356–61.doi:10.1006/bbrc.1995.2137.PMID7639757.
- Takahashi S, Kasai K, Hatsuzawa K, Kitamura N, Misumi Y, Ikehara Y, et al. (September 1993). "A mutation of furin causes the lack of precursor-processing activity in human colon carcinoma LoVo cells".Biochemical and Biophysical Research Communications.195(2): 1019–26.doi:10.1006/bbrc.1993.2146.PMID7690548.
- Hendy GN, Bennett HP, Gibbs BF, Lazure C, Day R, Seidah NG (April 1995)."Proparathyroid hormone is preferentially cleaved to parathyroid hormone by the prohormone convertase furin. A mass spectrometric study".The Journal of Biological Chemistry.270(16): 9517–25.doi:10.1074/jbc.270.16.9517.PMID7721880.
- Dubois CM, Laprise MH, Blanchette F, Gentry LE, Leduc R (May 1995)."Processing of transforming growth factor beta 1 precursor by human furin convertase".The Journal of Biological Chemistry.270(18): 10618–24.doi:10.1074/jbc.270.18.10618.PMID7737999.
- Gu M, Rappaport J, Leppla SH (May 1995). "Furin is important but not essential for the proteolytic maturation of gp160 of HIV-1".FEBS Letters.365(1): 95–7.doi:10.1016/0014-5793(95)00447-H.PMID7774724.S2CID21231590.
- Schäfer W, Stroh A, Berghöfer S, Seiler J, Vey M, Kruse ML, et al. (June 1995)."Two independent targeting signals in the cytoplasmic domain determine trans-Golgi network localization and endosomal trafficking of the proprotein convertase furin".The EMBO Journal.14(11): 2424–35.doi:10.1002/j.1460-2075.1995.tb07240.x.PMC398356.PMID7781597.
- Mbikay M, Seidah NG, Chrétien M, Simpson EM (March 1995). "Chromosomal assignment of the genes for proprotein convertases PC4, PC5, and PACE 4 in mouse and human".Genomics.26(1): 123–9.doi:10.1016/0888-7543(95)80090-9.PMID7782070.