Jacalin
Jacalinis a plant-basedlectin,but not alegume lectin,found injackfruit.It has been studied for capturing O-glycoproteinssuch asmucinsandIgA1, for potential applications in humanimmunology.[1]
Jacalin belongs to a family of galactose-binding lectins containing theJacalin-like lectin domainand it has a tetrameric two-chain structure with a weight of 66 kDa
Jacalin is preferably used in applications to isolate IgA from human serum, isolating human plasma glycoproteins and for applications in histochemistry. The lectin is blood group non-specific after neuraminidase treatment and agglutinates human erythrocytes at a concentration of ≥ 7,8 μg/mL.
A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving theN-terminusof the a-chain. The relative affinities of the lectin for galactose derivatives, as well as the structural basis of its T-antigen specificity, are explained by its protein structure[2]
See also
[edit]References
[edit]- ^Glycobiology:Elucidation of binding specificity of Jacalin toward O-glycosylated peptides: quantitative analysis by frontal affinity chromatography
- ^"Jacalin specification sheet Medicago AB: Jacalin"(PDF).Archived fromthe original(PDF)on March 18, 2012.RetrievedMarch 14,2010.