Sakaguchi test
Appearance
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TheSakaguchi testis achemical testused to detect presence ofargininein proteins. It is named after the Japanese food scientist and organic chemist, Shoyo Sakaguchi (1900–1995) who described the test in 1925.[1]The Sakaguchi reagent used in the test consists of1-Naphtholand a drop ofsodium hypobromite.Theguanidino(–C group in arginine reacts with the Sakaguchi reagent to form a red-coloured complex.[2][3][4][5]
References
[edit]- ^Sakaguchi, Shoyo (April 1925)."Über eine neue Farbenreaktion von protein und arginin"[About a new colorimetric reaction of protein and arginine].Journal of Biochemistry(in German).5.Oxford University Press;Japanese Biochemical Society:25–31.doi:10.1093/oxfordjournals.jbchem.a128359.
- ^Chatterjea (1 January 2004).Textbook of Biochemistry for Dental/Nursing/Pharmacy Students.Jaypee Brothers Publishers. p. 51.ISBN978-81-8061-204-6.
- ^R.A. Joshi (2006).Question Bank of Biochemistry.New Age International. p. 64.ISBN978-81-224-1736-4.
- ^Dandekar (1 January 2004).Practicals And Viva In Medical Biochemistry.Elsevier India. p. 28.ISBN978-81-8147-025-6.
- ^Joshi, Rashmi A.; Marks, Victor (30 June 2004).Guide to Bio-chemistry.B. Jain Publishers.p. 74.ISBN9788180560538.