Sakaguchi test

TheSakaguchi testis achemical testused to detect presence ofargininein proteins. It is named after the Japanese food scientist and organic chemist, Shoyo Sakaguchi (1900–1995) who described the test in 1925.^[1]The Sakaguchi reagent used in the test consists of1-Naphtholand a drop ofsodium hypobromite.Theguanidino(–C group in arginine reacts with the Sakaguchi reagent to form a red-coloured complex.^[2]^[3]^[4]^[5]

References

^Sakaguchi, Shoyo (April 1925)."Über eine neue Farbenreaktion von protein und arginin"[About a new colorimetric reaction of protein and arginine].Journal of Biochemistry(in German).5.Oxford University Press;Japanese Biochemical Society:25–31.doi:10.1093/oxfordjournals.jbchem.a128359.
^Chatterjea (1 January 2004).Textbook of Biochemistry for Dental/Nursing/Pharmacy Students.Jaypee Brothers Publishers. p. 51.ISBN 978-81-8061-204-6.
^R.A. Joshi (2006).Question Bank of Biochemistry.New Age International. p. 64.ISBN 978-81-224-1736-4.
^Dandekar (1 January 2004).Practicals And Viva In Medical Biochemistry.Elsevier India. p. 28.ISBN 978-81-8147-025-6.
^Joshi, Rashmi A.; Marks, Victor (30 June 2004).Guide to Bio-chemistry.B. Jain Publishers.p. 74.ISBN 9788180560538.

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[Sakaguchi1925-1] Sakaguchi, Shoyo (April 1925)."Über eine neue Farbenreaktion von protein und arginin"[About a new colorimetric reaction of protein and arginine].Journal of Biochemistry(in German).5.Oxford University Press;Japanese Biochemical Society:25–31.doi:10.1093/oxfordjournals.jbchem.a128359.

[2] Chatterjea (1 January 2004).Textbook of Biochemistry for Dental/Nursing/Pharmacy Students.Jaypee Brothers Publishers. p. 51.ISBN 978-81-8061-204-6.

[3] R.A. Joshi (2006).Question Bank of Biochemistry.New Age International. p. 64.ISBN 978-81-224-1736-4.

[4] Dandekar (1 January 2004).Practicals And Viva In Medical Biochemistry.Elsevier India. p. 28.ISBN 978-81-8147-025-6.

[5] Joshi, Rashmi A.; Marks, Victor (30 June 2004).Guide to Bio-chemistry.B. Jain Publishers.p. 74.ISBN 9788180560538.

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