Steroid 15beta-monooxygenase
| Steroid 15beta-monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.15.8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDBstructures | RCSB PDBPDBePDBsum | ||||||||
| |||||||||
Steroid 15beta-monooxygenase(EC1.14.15.8,cytochrome P-450meg,cytochrome P450meg,steroid 15beta-hydroxylase,CYP106A2,BmCYP106A2) is anenzymewithsystematic nameprogesterone,reduced-ferredoxin:oxygen oxidoreductase (15beta-hydroxylating).[1][2][3][4][5]This enzymecatalysesthe followingchemical reaction
- progesterone+ reduced ferredoxin + O215beta-hydroxyprogesterone+ oxidizedferredoxin+ H2O
The enzyme fromBacillus megateriumhydroxylates a variety of 3-oxo-Delta4-steroids in position 15beta.
References
[edit]- ^Berg A, Ingelman-Sundberg M, Gustafsson JA (June 1979)."Purification and characterization of cytochrome P-450meg".The Journal of Biological Chemistry.254(12): 5264–71.doi:10.1016/S0021-9258(18)50589-5.PMID109432.
- ^Berg A, Gustafsson JA, Ingelman-Sundberg M (May 1976)."Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium".The Journal of Biological Chemistry.251(9): 2831–8.doi:10.1016/S0021-9258(17)33564-0.PMID177422.
- ^Lisurek M, Kang MJ, Hartmann RW, Bernhardt R (June 2004). "Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry".Biochemical and Biophysical Research Communications.319(2): 677–82.doi:10.1016/j.bbrc.2004.05.037.PMID15178459.
- ^Goñi G, Zöllner A, Lisurek M, Velázquez-Campoy A, Pinto S, Gómez-Moreno C, Hannemann F, Bernhardt R, Medina M (November 2009). "Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368".Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics.1794(11): 1635–42.doi:10.1016/j.bbapap.2009.07.012.PMID19635596.
- ^Lisurek M, Simgen B, Antes I, Bernhardt R (June 2008). "Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation".ChemBioChem.9(9): 1439–49.doi:10.1002/cbic.200700670.PMID18481342.S2CID10667895.
External links
[edit]- Steroid+15beta-monooxygenaseat the U.S. National Library of MedicineMedical Subject Headings(MeSH)
