60S ribosomal protein L5
60S ribosomal protein L5is aproteinthat in humans is encoded by theRPL5gene.[5][6]
Function
[edit]Ribosomes,theorganellesthat catalyzeprotein synthesis,consist of a small40Ssubunit and a large60Ssubunit. Together these subunits are composed of 4RNAspecies and approximately 80 structurally distinct proteins. This gene encodes aribosomal proteinthat is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in thecytoplasm.The protein binds5S rRNAto form a stable complex called the 5Sribonucleoprotein particle(RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to thenucleolusfor assembly into ribosomes. The protein interacts specifically with the beta subunit ofcasein kinase 2.
Clinical significance
[edit]Variable expression of this gene incolorectal cancerscompared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with thesmall nucleolar RNAgeneU21,which is located in its fifthintron.As is typical for genes encoding ribosomal proteins, there are multipleprocessed pseudogenesof this gene dispersed through the genome.[7]
Interactions
[edit]Ribosomal protein L5 has been shown tointeractwith:
References
[edit]- ^abcGRCh38: Ensembl release 89: ENSG00000122406–Ensembl,May 2017
- ^abcGRCm38: Ensembl release 89: ENSMUSG00000058558–Ensembl,May 2017
- ^"Human PubMed Reference:".National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^"Mouse PubMed Reference:".National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^Qu LH, Nicoloso M, Michot B, Azum MC, Caizergues-Ferrer M, Renalier MH, Bachellerie JP (November 1994)."U21, a novel small nucleolar RNA with a 13 nt. complementarity to 28S rRNA, is encoded in an intron of ribosomal protein L5 gene in chicken and mammals".Nucleic Acids Res.22(20): 4073–81.doi:10.1093/nar/22.20.4073.PMC331892.PMID7937132.
- ^Frigerio JM, Dagorn JC, Iovanna JL (July 1995). "Cloning, sequencing and expression of the L5, L21, L27a, L28, S5, S9, S10 and S29 human ribosomal protein mRNAs".Biochim Biophys Acta.1262(1): 64–8.doi:10.1016/0167-4781(95)00045-i.PMID7772601.
- ^"Entrez Gene: RPL5 ribosomal protein L5".
- ^Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM (January 2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region".Genomics.83(1): 153–67.doi:10.1016/s0888-7543(03)00235-0.PMID14667819.
- ^Boldyreff B, Issinger OG (February 1997). "A-Raf kinase is a new interacting partner of protein kinase CK2 beta subunit".FEBS Lett.403(2): 197–9.doi:10.1016/s0014-5793(97)00010-0.PMID9042965.S2CID84557809.
- ^Kim JM, Cha JY, Marshak DR, Bae YS (September 1996)."Interaction of the beta subunit of casein kinase II with the ribosomal protein L5".Biochem. Biophys. Res. Commun.226(1): 180–6.doi:10.1006/bbrc.1996.1330.PMID8806611.
- ^Ahn BH, Kim TH, Bae YS (October 2001)."Mapping of the interaction domain of the protein kinase CKII beta subunit with target proteins".Mol. Cells.12(2): 158–63.doi:10.1016/S1016-8478(23)17077-4.PMID11710515.
- ^Schatz O, Oft M, Dascher C, Schebesta M, Rosorius O, Jaksche H, Dobrovnik M, Bevec D, Hauber J (February 1998)."Interaction of the HIV-1 rev cofactor eukaryotic initiation factor 5A with ribosomal protein L5".Proc. Natl. Acad. Sci. U.S.A.95(4): 1607–12.Bibcode:1998PNAS...95.1607S.doi:10.1073/pnas.95.4.1607.PMC19115.PMID9465063.
- ^Zhang Y, Wolf GW, Bhat K, Jin A, Allio T, Burkhart WA, Xiong Y (Dec 2003)."Ribosomal protein L11 negatively regulates oncoprotein MDM2 and mediates a p53-dependent ribosomal-stress checkpoint pathway".Mol. Cell. Biol.23(23): 8902–12.doi:10.1128/mcb.23.23.8902-8912.2003.PMC262682.PMID14612427.
- ^Marechal V, Elenbaas B, Piette J, Nicolas JC, Levine AJ (November 1994)."The ribosomal L5 protein is associated with mdm-2 and mdm-2-p53 complexes".Mol. Cell. Biol.14(11): 7414–20.doi:10.1128/mcb.14.11.7414.PMC359276.PMID7935455.
- ^Dai MS, Sun XX, Lu H (July 2008)."Aberrant expression of nucleostemin activates p53 and induces cell cycle arrest via inhibition of MDM2".Mol. Cell. Biol.28(13): 4365–76.doi:10.1128/MCB.01662-07.PMC2447154.PMID18426907.
- ^Kang MJ, Ahn HS, Lee JY, Matsuhashi S, Park WY (April 2002). "Up-regulation of PDCD4 in senescent human diploid fibroblasts".Biochem. Biophys. Res. Commun.293(1): 617–21.doi:10.1016/S0006-291X(02)00264-4.PMID12054647.
Further reading
[edit]- Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal proteins".Biochem. Cell Biol.73(11–12): 933–47.doi:10.1139/o95-101.PMID8722009.
- Pogue-Geile K, Geiser JR, Shu M, Miller C, Wool IG, Meisler AI,Pipas JM(1991)."Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein".Mol. Cell. Biol.11(8): 3842–9.doi:10.1128/MCB.11.8.3842.PMC361167.PMID1712897.
- Steitz JA, Berg C, Hendrick JP, La Branche-Chabot H, Metspalu A, Rinke J, Yario T (1988)."A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells".J. Cell Biol.106(3): 545–56.doi:10.1083/jcb.106.3.545.PMC2115095.PMID3279045.
- Hirano K, Ito M, Hartshorne DJ (1995)."Interaction of the ribosomal protein, L5, with protein phosphatase type 1".J. Biol. Chem.270(34): 19786–90.doi:10.1074/jbc.270.34.19786.PMID7649987.
- Marechal V, Elenbaas B, Piette J, Nicolas JC, Levine AJ (1994)."The ribosomal L5 protein is associated with mdm-2 and mdm-2-p53 complexes".Mol. Cell. Biol.14(11): 7414–20.doi:10.1128/mcb.14.11.7414.PMC359276.PMID7935455.
- Ogata K, Kurahashi A, Nishiyama C, Terao K (1994). "Presence of role of the 5SrRNA-L5 protein complex (5SRNP) in the threonyl- and histidyl-tRNA synthetase complex in rat liver cytosol".Biochim. Biophys. Acta.1218(3): 388–400.doi:10.1016/0167-4781(94)90192-9.PMID8049265.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides".Gene.138(1–2): 171–4.doi:10.1016/0378-1119(94)90802-8.PMID8125298.
- Michael WM, Dreyfuss G (1996)."Distinct domains in ribosomal protein L5 mediate 5 S rRNA binding and nucleolar localization".J. Biol. Chem.271(19): 11571–4.doi:10.1074/jbc.271.19.11571.PMID8626719.
- Kim JM, Cha JY, Marshak DR, Bae YS (1996)."Interaction of the beta subunit of casein kinase II with the ribosomal protein L5".Biochem. Biophys. Res. Commun.226(1): 180–6.doi:10.1006/bbrc.1996.1330.PMID8806611.
- Boldyreff B, Issinger OG (1997). "A-Raf kinase is a new interacting partner of protein kinase CK2 beta subunit".FEBS Lett.403(2): 197–9.doi:10.1016/S0014-5793(97)00010-0.PMID9042965.S2CID84557809.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library".Gene.200(1–2): 149–56.doi:10.1016/S0378-1119(97)00411-3.PMID9373149.
- Schatz O, Oft M, Dascher C, Schebesta M, Rosorius O, Jaksche H, Dobrovnik M, Bevec D, Hauber J (1998)."Interaction of the HIV-1 rev cofactor eukaryotic initiation factor 5A with ribosomal protein L5".Proc. Natl. Acad. Sci. U.S.A.95(4): 1607–12.Bibcode:1998PNAS...95.1607S.doi:10.1073/pnas.95.4.1607.PMC19115.PMID9465063.
- Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (1998)."A map of 75 human ribosomal protein genes".Genome Res.8(5): 509–23.doi:10.1101/gr.8.5.509.PMID9582194.
- Jäkel S, Görlich D (1998)."Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells".EMBO J.17(15): 4491–502.doi:10.1093/emboj/17.15.4491.PMC1170780.PMID9687515.
- Guerra B, Issinger OG (1998). "p53 and the ribosomal protein L5 participate in high molecular mass complex formation with protein kinase CK2 in murine teratocarcinoma cell line F9 after serum stimulation and cisplatin treatment".FEBS Lett.434(1–2): 115–20.doi:10.1016/S0014-5793(98)00962-4.PMID9738462.S2CID7694535.
- Park JW, Bae YS (1999). "Phosphorylation of ribosomal protein L5 by protein kinase CKII decreases its 5S rRNA binding activity".Biochem. Biophys. Res. Commun.263(2): 475–81.doi:10.1006/bbrc.1999.1345.PMID10491318.
- Rosorius O, Fries B, Stauber RH, Hirschmann N, Bevec D, Hauber J (2000)."Human ribosomal protein L5 contains defined nuclear localization and export signals".J. Biol. Chem.275(16): 12061–8.doi:10.1074/jbc.275.16.12061.PMID10766838.