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Ceramide

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Ceramide. R represents the alkyl portion of a fatty acid.
General structures ofsphingolipids

Ceramidesare a family ofwaxylipidmolecules. A ceramide is composed ofsphingosineand afatty acidjoined by anamide bond.Ceramides are found in high concentrations within thecell membraneofeukaryoticcells, since they are component lipids that make upsphingomyelin,one of the major lipids in thelipid bilayer.[1]Contrary to previous assumptions that ceramides and othersphingolipidsfound in cell membrane were purely supporting structural elements, ceramide can participate in a variety of cellularsignaling:examples include regulatingdifferentiation,proliferation,andprogrammed cell death(PCD) ofcells.

The wordceramidecomes from the Latincera(wax) andamide.Ceramide is a component ofvernix caseosa,the waxy or cheese-like white substance found coating the skin of newborn human infants.

Pathways for ceramide synthesis[edit]

There are three major pathways of ceramide generation. First, the sphingomyelinase pathway uses an enzyme to break down sphingomyelin in the cell membrane and release ceramide. Second, thede novopathway creates ceramide from less complex molecules. Third, in the "salvage" pathway, sphingolipids that are broken down intosphingosineare reused byreacylationto form ceramide.

Sphingomyelin hydrolysis[edit]

Hydrolysisofsphingomyelinis catalyzed by the enzymesphingomyelinase.Because sphingomyelin is one of the four commonphospholipidsfound in theplasma membraneof cells, the implications of this method of generating ceramide is that the cellular membrane is the target of extracellular signals leading to programmed cell death. There has been research suggesting that when ionizingradiationcausesapoptosisin some cells, the radiation leads to the activation of sphingomyelinase in the cell membrane and ultimately, to ceramide generation.[2]

De novo[edit]

De novo synthesis of ceramide begins with the condensation ofpalmitateandserineto form 3-keto-dihydrosphingosine. This reaction is catalyzed by the enzymeserine palmitoyl transferaseand is the rate-limiting step of the pathway. In turn, 3-keto-dihydrosphingosine is reduced todihydrosphingosine,which is then followed by acylation by the enzyme (dihydro)ceramide synthase to producedihydroceramide.The final reaction to produce ceramide is catalyzed bydihydroceramide desaturase.De novo synthesis of ceramide occurs in theendoplasmic reticulum.Ceramide is subsequently transported to theGolgi apparatusby either vesicular trafficking or the ceramide transfer protein CERT. Once in the Golgi apparatus, ceramide can be further metabolized to othersphingolipids,such assphingomyelinand the complexglycosphingolipids.[3]

Salvage pathway[edit]

Constitutive degradation of sphingolipids and glycosphingolipids takes place in the acidic subcellular compartments, the late endosomes and thelysosomes,with the end goal of producing sphingosine. In the case of glycosphingolipids, exohydrolases acting at acidic pH optima cause the stepwise release of monosaccharide units from the end of the oligosaccharide chains, leaving just the sphingosine portion of the molecule, which may then contribute to the generation of ceramides. Ceramide can be further hydrolyzed by acid ceramidase to form sphingosine and a free fatty acid, both of which are able to leave the lysosome, unlike ceramide. The long-chain sphingoid bases released from the lysosome may then re-enter pathways for synthesis of ceramide and/orsphingosine-1-phosphate.The salvage pathway re-utilizes long-chain sphingoid bases to form ceramide through the action of ceramide synthase. Thus,ceramide synthasefamily members probably trap free sphingosine released from the lysosome at the surface of theendoplasmic reticulumor in endoplasmic reticulum-associated membranes. The salvage pathway has been estimated to contribute from 50% to 90% of sphingolipid biosynthesis.[4]

Physiological roles[edit]

Pathology[edit]

As a bioactive lipid, ceramide has been implicated in a variety of physiological functions includingapoptosis,cell growth arrest, differentiation, cellsenescence,cell migration and adhesion.[3]Roles for ceramide and its downstream metabolites have also been suggested in a number of pathological states includingcancer,neurodegeneration,diabetes,microbial pathogenesis,obesity,andinflammation.[5][6]

Several distinct ceramides potently predictmajor adverse cardiovascular events(MACE), namely C16:0, C18:0, and C24:1, although C24:0 has an inverse relationship.[7][8]C16-C18 are harmful in the liver.[7]Ceramide levels are positively correlated with inflammation and oxidative stress in the liver, and the onset and progression ofnon-alcoholic fatty liver disease(NAFLD) is associated with elevated ceramide inhepatocytes.[8]Dietary intake ofsaturated fathas been shown to increase serum ceramide and increaseinsulin resistance.[7]Although initial studies showed increased insulin resistance in muscle, subsequent studies also showed increased insulin resistance in liver andadipose tissue.[8]Interventions that limit ceramide synthesis or increase ceramide degradation lead to improved health (reduced insulin resistance and reducedfatty liver disease,for example).[7]

Ceramides induce skeletal muscle insulin resistance when synthesized as a result of saturated fat activation ofTLR4receptors.[9]Unsaturated fatdoes not have this effect.[9]Ceramides induce insulin resistance in many tissues by inhibition ofAkt/PKB signaling.[10]Aggregation ofLDL cholesterolby ceramide causes LDL retention in arterial walls, leading toatherosclerosis.[11]Ceramides causeendothelial dysfunctionby activatingprotein phosphatase 2(PP2A).[12]In mitochondria, ceramide suppresses theelectron transport chainand induces production ofreactive oxygen species.[13]

Apoptosis[edit]

One of the most studied roles of ceramide pertains to its function as a proapoptotic molecule.Apoptosis,or Type Iprogrammed cell death,is essential for the maintenance of normal cellular homeostasis and is an important physiological response to many forms of cellular stress. Ceramide accumulation has been found following treatment of cells with a number of apoptotic agents, including ionizing radiation,[2][14]UVlight,[15]TNF- Alpha,[16]andchemotherapeutic agents.This suggests a role for ceramide in the biological responses of all these agents. Because of its apoptosis-inducing effects in cancer cells, ceramide has been termed the "tumor suppressor lipid". Several studies have attempted to define further the specific role of ceramide in the events of cell death and some evidence suggests ceramide functions upstream of themitochondriain inducing apoptosis. However, owing to the conflicting and variable nature of studies into the role of ceramide in apoptosis, the mechanism by which this lipid regulates apoptosis remains elusive.[17]

Skin[edit]

Thestratum corneumis the outermost layer of theepidermis.[18][19][20]It is composed of terminally differentiated and enucleated corneocytes that reside within a lipid matrix, like "bricks and mortar." Together withcholesteroland free fatty acids, ceramides form the lipid mortar, a water-impermeable barrier that prevents evaporative water loss. As a general rule of thumb, the epidermal lipid matrix is composed of an equimolar mixture of ceramides (~50% by weight), cholesterol (~ 25% by weight), and free fatty acids (~15% by weight), with smaller quantities of other lipids also being present.[21][22]The lipid barrier also protects against the entry of microorganisms.[20]

Epidermal ceramides have a diversity of structures and can be broadly classified as AS and NS ceramides; ADS and NDS dihydroceramides; AH, EOH, and NH 6-hydroxyceramides; AP and NP phytoceramides; and EOH and EOS acylceramides, see figure.

Epidermal Ceramides. (Merleev et. al., JCI Insight 2022, Supplemental Data p.14- Supplemental Fig. 1)

[18]The diversity of ceramide structures undoubtedly plays an important role in the unique attributes of the stratum corneum across different body sites. For example, the stratum corneum of the face is thin and flexible to accommodate different facial expressions. In contrast, the stratum corneum covering the heel of the foot is thick and rigid to protect against trauma. Matching these structural changes, there are body-site specific alterations in the epidermal lipidome, including changes in the relative abundance of the different epidermal ceramide structures.[18]

Similar to body site-specific alterations in ceramide abundance, there are also well-characterized changes in epidermal ceramide expression in patients with inflammatory skin diseases. In thehyperplasticdisorderpsoriasis,investigators have reported an increase in AS and NS ceramides and a decrease in EOS, AP, and NP ceramides, which may contribute to a defect in the skin's water impermeability barrier.[23][24][22]Studying ceramide expression inatopic dermatitisandpsoriasispatients, other investigators have reported that rather than focusing on ceramide classes, ceramide sphingoid base length and fatty acid chain length have the strongest influence on the likelihood of a particular ceramide structure being upregulated or downregulated in inflamed skin.[18]

Hormonal[edit]

Inhibition of ceramide synthesis withmyriocinin obese mice may lead to both improvedleptinsignaling and decreasedinsulin resistanceby decreasingSOCS-3expression.[25]An elevated level of ceramide can cause insulin resistance by inhibiting the ability of insulin to activate theinsulin signal transduction pathwayand/or via the activation ofJNK.[26]

Substances known to induce ceramide generation[edit]

Mechanism by which ceramide signaling occurs[edit]

Currently, the means by which ceramide acts as a signaling molecule are not clear.

One hypothesis is that ceramide generated in theplasma membraneenhancesmembrane rigidityand stabilizes smaller lipid platforms known aslipid rafts,allowing them to serve as platforms for signalling molecules. Moreover, as rafts on one leaflet of the membrane can induce localized changes in the other leaflet of the bilayer, they can potentially serve as the link between signals from outside the cell to signals to be generated within the cell.

Ceramide has also been shown to form organized large channels traversing the mitochondrial outer membrane. This leads to the egress of proteins from the intermembrane space.[32][33][34]

Related diseases[edit]

In the metabolic diseasecombined malonic and methylmalonic aciduria(CMAMMA) due toACSF3,a massive altered composition of complex lipids occurs as a result of impaired mitochondrialfatty acid synthesis(mtFAS).[35][36]For example, while the concentration ofsphingomyelinis noticeably increased, the concentration of ceramides is proportionally decreased.[35]

Uses[edit]

Ceramides may be found as ingredients of some topical skin medications used to complement treatment for skin conditions such aseczema.[37]They are also used in cosmetic products such as some soaps, shampoos, skin creams, and sunscreens.[38]Additionally, ceramides are being explored as a potential therapeutic in treating cancer.[39]

Ceramide in bacteria[edit]

Ceramide is rarely found in bacteria.[40]Bacteria of familySphingomonadaceae,however, contain it.

Ceramide phosphoethanolamine[edit]

Ceramide phosphoethanolamine (CPE) is asphingolipidconsisted of a ceramide and aphosphoethanolaminehead group. CPE is the major sphingolipid class in some invertebrates such as members ofDrosophila.In contrast, mammalian cells contain only small amounts of CPE.

References[edit]

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External links[edit]

Scholiahas achemical-classprofile forCeramide.
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