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Human Genome Project

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Logo of the Human Genome Project

TheHuman Genome Project(HGP) was an internationalscientific researchproject with the goal of determining thebase pairsthat make up humanDNA,and of identifying,mappingandsequencingall of thegenesof thehuman genomefrom both a physical and a functional standpoint. It started in 1990 and was completed in 2003.[1]It remains the world's largest collaborative biological project.[2]Planning for the project started after it was adopted in 1984 by theUS government,and it officially launched in 1990. It was declared complete on April 14, 2003, and included about 92% of the genome.[3]Level "complete genome" was achieved in May 2021, with a remaining only 0.3% bases covered by potential issues.[4][5]The final gapless assembly was finished in January 2022.[6]

Funding came from the United States government through theNational Institutes of Health(NIH) as well as numerous other groups from around the world. A parallel project was conducted outside the government by theCelera Corporation,or Celera Genomics, which was formally launched in 1998. Most of the government-sponsored sequencing was performed in twenty universities and research centres in theUnited States,theUnited Kingdom,Japan,France,Germany,andChina,[7]working in the International Human Genome Sequencing Consortium (IHGSC).

The Human Genome Project originally aimed to map the complete set ofnucleotidescontained in a humanhaploidreference genome,of which there are more than three billion. The "genome" of any given individual is unique; mapping the "human genome" involved sequencing samples collected from a small number of individuals and then assembling the sequenced fragments to get a complete sequence for each of the 23 human chromosomes pairs (22 pairs of autosomes and a pair of sex chromosomes, known as allosomes). Therefore, the finished human genome is a mosaic, not representing any one individual. Much of the project's utility comes from the fact that the vast majority of the human genome is the same in all humans.

History[edit]

The Human Genome Project was a 15 year-long publicly funded project initiated in 1990 with the objective of determining the DNA sequence of the entireeuchromatichuman genome within 13 years.[8][9]The idea of such a project originated in the work ofRonald A. Fisher,whose work is also credited with later initiating the project.[10]

In May 1985, Robert Sinsheimer organized a workshop at theUniversity of California, Santa Cruz,to discuss the feasibility of building a systematicreference genomeusing gene sequencing technologies.[11]In March 1986, the Santa Fe Workshop was organized byCharles DeLisiand David Smith of theDepartment of Energy's Office of Health and Environmental Research (OHER).[12]At the same timeRenato Dulbecco,President of theSalk Institute for Biological Studies,first proposed the concept ofwhole genome sequencingin an essay inScience.[13]The published work, titled "A Turning Point in Cancer Research: Sequencing the Human Genome", was shortened from the original proposal of using the sequence to understand the genetic basis of breast cancer.[14]James Watson,one of the discoverers of the double helix shape of DNA in the 1950s, followed two months later with a workshop held at the Cold Spring Harbor Laboratory. Thus the idea for obtaining a reference sequence had three independent origins: Sinsheimer, Dulbecco and DeLisi. Ultimately it was the actions by DeLisi that launched the project.[15][16][17][18]

The fact that the Santa Fe Workshop was motivated and supported by a federal agency opened a path, albeit a difficult and tortuous one,[19]for converting the idea into public policy in the United States. In a memo to the Assistant Secretary for Energy ResearchAlvin Trivelpiece,then-Director of the OHER Charles DeLisi outlined a broad plan for the project.[20]This started a long and complex chain of events which led to approved reprogramming of funds that enabled the OHER to launch the project in 1986, and to recommend the first line item for the HGP, which was in President Reagan's 1988 budget submission,[19]and ultimately approved by Congress. Of particular importance in congressional approval was the advocacy of New Mexico SenatorPete Domenici,whom DeLisi had befriended.[21]Domenici chaired the Senate Committee on Energy and Natural Resources, as well as the Budget Committee, both of which were key in the DOE budget process. Congress added a comparable amount to the NIH budget, thereby beginning official funding by both agencies.[citation needed]

Trivelpiece sought and obtained the approval of DeLisi's proposal by Deputy SecretaryWilliam Flynn Martin.This chart[22]was used by Trivelpiece in the spring of 1986 to brief Martin and Under Secretary Joseph Salgado regarding his intention to reprogram $4 million to initiate the project with the approval ofJohn S. Herrington.[citation needed]This reprogramming was followed by a line item budget of $13 million in theReagan administration's 1987 budget submission to Congress.[12]It subsequently passed both Houses. The project was planned to be completed within 15 years.[23]

Proposal toWilliam F. Martinlaunching the Human Genome Project

In 1990, the two major funding agencies, DOE and theNational Institutes of Health,developed a memorandum of understanding in order to coordinate plans and set the clock for the initiation of the Project to 1990.[24]At that time, David J. Galas was Director of the renamed "Office of Biological and Environmental Research" in the U.S. Department of Energy's Office of Science andJames Watsonheaded the NIH Genome Program. In 1993,Aristides Patrinossucceeded Galas andFrancis Collinssucceeded Watson, assuming the role of overall Project Head as Director of the NIH National Center for Human Genome Research (which would later become theNational Human Genome Research Institute). A working draft of the genome was announced in 2000 and the papers describing it were published in February 2001. A more complete draft was published in 2003, and genome "finishing" work continued for more than a decade after that.[citation needed]

The $3 billion project was formally founded in 1990 by the US Department of Energy and the National Institutes of Health, and was expected to take 15 years.[25]In addition to the United States, the internationalconsortiumcomprisedgeneticistsin the United Kingdom, France, Australia, China, and myriad other spontaneous relationships.[26]The project ended up costing less than expected, at about $2.7 billion (equivalent to about $5 billion in 2021).[7][27][28]

Two technologies enabled the project:gene mappingandDNA sequencing.The gene mapping technique ofrestriction fragment length polymorphism(RFLP) arose from the search for the location of the breast cancer gene by Mark Skolnick of the University of Utah,[29]which began in 1974.[30]Seeing a linkage marker for the gene, collaboration withDavid Botstein,Ray Whiteand Ron Davies conceived of a way to construct agenetic linkagemap of the human genome. This enabled scientists to launch the larger human genome effort.[31]

Because of widespread international cooperation and advances in the field ofgenomics(especially insequence analysis), as well as parallel advances in computing technology, a 'rough draft' of the genome was finished in 2000 (announced jointly by U.S. PresidentBill Clintonand BritishPrime MinisterTony Blairon June 26, 2000).[32][33]This first available rough draftassemblyof the genome was completed by the Genome Bioinformatics Group at theUniversity of California, Santa Cruz,primarily led by then-graduate studentJim Kentand his advisorDavid Haussler.[34]Ongoing sequencing led to the announcement of the essentially complete genome on April 14, 2003, two years earlier than planned.[35][36]In May 2006, another milestone was passed on the way to completion of the project when the sequence of thevery last chromosomewas published inNature.[37]

The various institutions, companies, and laboratories which participated in the Human Genome Project are listed below, according to theNIH:[7]

No. Nation Name Affiliation
1 United States The Whitehead Institute/MIT Center for Genome Research Massachusetts Institute of Technology
2 United Kingdom The Wellcome Trust Sanger Institute Wellcome Trust
3 United States Washington University School of Medicine Genome Sequencing Center Washington University in St. Louis
4 United States United States DOE Joint Genome Institute United States Department of Energy
5 United States Baylor College of Medicine Human Genome Sequencing Center Baylor College of Medicine
6 Japan RIKEN Genomic Sciences Center Riken
7 France Genoscope and CNRS UMR-8030 French Alternative Energies and Atomic Energy Commission
8 United States GTC Sequencing Center Genome Therapeutics Corporation,whose sequencing division is acquired byABI
9 Germany Department of Genome Analysis Fritz Lipmann Institute, name changed from Institute of Molecular Biotechnology
10 China Beijing Genomics Institute/Human Genome Center Chinese Academy of Sciences
11 United States Multimegabase Sequencing Center Institute for Systems Biology
12 United States Stanford Genome Technology Center Stanford University
13 United States Stanford Human Genome Center and Department of Genetics Stanford University School of Medicine
14 United States University of Washington Genome Center University of Washington
15 Japan Department of Molecular Biology Keio UniversitySchool of Medicine
16 United States University of Texas Southwestern Medical Center at Dallas University of Texas
17 United States University of Oklahoma's Advanced Center for Genome Technology Dept. of Chemistry and Biochemistry,University of Oklahoma
18 Germany Max Planck Institute for Molecular Genetics Max Planck Society
19 United States Lita Annenberg Hazen Genome Center Cold Spring Harbor Laboratory
20 Germany GBF/German Research Centre for Biotechnology Reorganized and renamed toHelmholtz Centre for Infection Research

State of completion[edit]

Notably, the project was not able to sequence all of the DNA found inhuman cells;rather, the aim was to sequence onlyeuchromaticregions of the nuclear genome, which make up 92.1% of the human genome. The remaining 7.9% exists in scatteredheterochromaticregions such as those found incentromeresandtelomeres.These regions by their nature are generally more difficult to sequence and so were not included as part of the project's original plans.[38]

The Human Genome Project (HGP) was declared complete in April 2003. An initial rough draft of the human genome was available in June 2000 and by February 2001 a working draft had been completed and published followed by the final sequencing mapping of the human genome on April 14, 2003. Although this was reported to cover 99% of the euchromatic human genome with 99.99% accuracy, a major quality assessment of the human genome sequence was published on May 27, 2004, indicating over 92% of sampling exceeded 99.99% accuracy which was within the intended goal.[39]

In March 2009, theGenome Reference Consortium(GRC) released a more accurate version of the human genome, but that still left more than 300 gaps,[40]while 160 such gaps remained in 2015.[41]

Though in May 2020, the GRC reported 79 "unresolved" gaps,[42]accounting for as much as 5% of the human genome,[43]months later, the application of newlong-range sequencing techniquesand ahydatidiform mole-derived cell line in which both copies of each chromosome are identical led to the first telomere-to-telomere, truly complete sequence of a human chromosome, theX chromosome.[44]Similarly, an end-to-end complete sequence of human autosomalchromosome 8followed several months later.[45]

In 2021, it was reported that the Telomere-to-Telomere (T2T) consortium had filled in all of the gaps except five in repetitive regions of ribosomal DNA.[46]Months later, those gaps had also been closed. The full sequence did not contain theY chromosome,which causes the embryo to become male, being absent in the cell line that served as the source for the DNA analyzed. About 0.3% of the full sequence proved difficult to check for quality, and thus might have contained errors,[47]which were being targeted for confirmation.[48]In April 2022, the complete non-Y chromosome sequence was formally published, providing a view of much of the 8% of the genome left out by the HGP.[49]In December, 2022, apreprintarticle claimed that the sequencing of the remaining missing regions of Y chromosome had been performed, thus completing the sequencing of all 24 human chromosomes.[50]In August 2023 this preprint was finally published.[51][52]

Applications and proposed benefits[edit]

The sequencing of the human genome holds benefits for many fields, frommolecular medicinetohuman evolution.The Human Genome Project, through its sequencing of the DNA, can help researchers understand diseases including:genotypingof specificvirusesto direct appropriate treatment; identification ofmutationslinked to different forms ofcancer;the design of medication and more accurate prediction of their effects; advancement inforensicapplied sciences;biofuelsand other energy applications;agriculture,animal husbandry,bioprocessing;risk assessment;bioarcheology,anthropologyandevolution. The sequence of the DNA is stored indatabasesavailable to anyone on theInternet.The U.S.National Center for Biotechnology Information(and sister organizations in Europe and Japan) house the gene sequence in a database known asGenBank,along with sequences of known and hypothetical genes and proteins. Other organizations, such as theUCSC Genome Browserat the University of California, Santa Cruz,[53]andEnsembl[54]present additional data and annotation and powerful tools for visualizing and searching it.Computer programshave been developed to analyze the data because the data itself is difficult to interpret without such programs. Generally speaking, advances in genome sequencing technology have followedMoore's Law,a concept from computer science which states that integrated circuits can increase in complexity at an exponential rate.[55]This means that the speeds at which whole genomes can be sequenced can increase at a similar rate, as was seen during the development of the Human Genome Project.

Techniques and analysis[edit]

The process of identifying the boundaries between genes and other features in a raw DNA sequence is calledgenome annotationand is in the domain ofbioinformatics.While expert biologists make the best annotators, their work proceeds slowly, and computer programs are increasingly used to meet the high-throughput demands of genome sequencing projects. Beginning in 2008, a new technology known asRNA-seqwas introduced that allowed scientists to directly sequence the messenger RNA in cells. This replaced previous methods of annotation, which relied on the inherent properties of the DNA sequence, with direct measurement, which was much more accurate. Today, annotation of the human genome and other genomes relies primarily on deep sequencing of the transcripts in every human tissue using RNA-seq. These experiments have revealed that over 90% of genes contain at least one and usually several alternative splice variants, in which theexonsare combined in different ways to produce 2 or more gene products from the same locus.[56]

The genome published by the HGP does not represent the sequence of every individual's genome. It is the combined mosaic of a small number of anonymous donors, of African, European and east Asian ancestry. The HGP genome is a scaffold for future work in identifying differences among individuals.[citation needed]Subsequent projects sequenced the genomes of multiple distinct ethnic groups, though as of 2019 there is still only one "reference genome".[57]

Findings[edit]

Key findings of the draft (2001) and complete (2004) genome sequences include:

  1. There are approximately 22,300[58]protein-coding genes in human beings, the same range as in other mammals.
  2. The human genome has significantly moresegmental duplications(nearly identical, repeated sections of DNA) than had been previously suspected.[59][60][61]
  3. At the time when the draft sequence was published, fewer than 7% ofprotein familiesappeared to be vertebrate specific.[62]

Accomplishments[edit]

The first printout of the human genome to be presented as a series of books, displayed at theWellcome Collection,London

The human genome has approximately 3.1 billionbase pairs.[63]The Human Genome Project was started in 1990 with the goal of sequencing and identifying all base pairs in the human genetic instruction set, finding the genetic roots of disease and then developing treatments. It is considered amegaproject.

The genome was broken into smaller pieces; approximately 150,000base pairsin length.[64]These pieces were then ligated into a type of vector known as "bacterial artificial chromosomes",or BACs, which are derived from bacterial chromosomes which have been genetically engineered. The vectors containing the genes can be inserted into bacteria where they are copied by the bacterialDNA replicationmachinery. Each of these pieces was then sequenced separately as a small "shotgun"project and then assembled. The larger, 150,000 base pairs go together to create chromosomes. This is known as the"hierarchical shotgun"approach, because the genome is first broken into relatively large chunks, which are then mapped to chromosomes before being selected for sequencing.[65][66]

Funding came from the US government through the National Institutes of Health in the United States, and a UK charity organization, theWellcome Trust,as well as numerous other groups from around the world. The funding supported a number of large sequencing centers including those atWhitehead Institute,theWellcome Sanger Institute(then called The Sanger Centre) based at theWellcome Genome Campus,Washington University in St. Louis,andBaylor College of Medicine.[25][67]

The United Nations Educational, Scientific and Cultural Organization (UNESCO) served as an important channel for the involvement of developing countries in the Human Genome Project.[68]

Public vis-à-vis private approaches[edit]

In 1998, a similar, privately funded quest was launched by the American researcherCraig Venter,and his firm Celera Genomics. Venter was a scientist at the NIH during the early 1990s when the project was initiated. The $300 million Celera effort was intended to proceed at a faster pace and at a fraction of the cost of the roughly $3 billionpublicly fundedproject. While the Celera project focused its efforts on production sequencing and assembly of the human genome, the public HGP also funded mapping and sequencing of theworm,fly,andyeastgenomes, funding of databases, development of new technologies, supporting bioinformatics and ethics programs, as well as polishing and assessment of the genome assembly.[69]Both the Celera and public approaches spent roughly $250 million on the production sequencing effort.[70]For sequence assembly, Celera made use of publicly available maps atGenBank,which Celera was capable of generating, but the availability of which was "beneficial" to the privately-funded project.[59]

Celera used a technique calledwhole genome shotgun sequencing,employingpairwise end sequencing,[71]which had been used to sequence bacterial genomes of up to six million base pairs in length, but not for anything nearly as large as the three billion base pair human genome.

Celera initially announced that it would seek patent protection on "only 200–300" genes, but later amended this to seeking "intellectual property protection" on "fully-characterized important structures" amounting to 100–300 targets. The firm eventually filed preliminary ( "place-holder" ) patent applications on 6,500 whole or partial genes. Celera also promised to publish their findings in accordance with the terms of the 1996 "Bermuda Statement",by releasing new data annually (the HGP released its new data daily), although, unlike the publicly funded project, they would not permit free redistribution or scientific use of the data. The publicly funded competitors were compelled to release the first draft of the human genome before Celera for this reason. On July 7, 2000, the UCSC Genome Bioinformatics Group released a first working draft on the web. The scientific community downloaded about 500 GB of information from the UCSC genome server in the first 24 hours of free and unrestricted access.[72]

In March 2000,President Clinton,along withPrime Minister Tony Blairin a dual statement, urged that all researchers who wished to research the sequence should have "unencumbered access" to the genome sequence.[73]The statement sent Celera's stock plummeting and dragged down thebiotechnology-heavyNasdaq.The biotechnology sector lost about $50 billion inmarket capitalizationin two days.[citation needed]

Although the working draft was announced in June 2000, it was not until February 2001 that Celera and the HGP scientists published details of their drafts. Special issues ofNature(which published the publicly funded project'sscientific paper)[59]described the methods used to produce the draft sequence and offered analysis of the sequence. These drafts covered about 83% of the genome (90% of the euchromatic regions with 150,000 gaps and the order and orientation of many segments not yet established). In February 2001, at the time of the joint publications,press releasesannounced that the project had been completed by both groups. Improved drafts were announced in 2003 and 2005, filling in to approximately 92% of the sequence currently.[citation needed]

Genome donors[edit]

In the International Human Genome Sequencing Consortium (IHGSC)public-sectorHGP, researchers collected blood (female) or sperm (male) samples from a large number of donors. Only a few of many collected samples were processed as DNA resources. Thus the donor identities were protected so neither donors nor scientists could know whose DNA was sequenced. DNA clones taken from many differentlibrarieswere used in the overall project, with most of those libraries being created by Pieter J. de Jong. Much of the sequence (>70%) of thereference genomeproduced by the public HGP came from a single anonymous male donor fromBuffalo, New York,(code nameRP11; the "RP" refers toRoswell Park Comprehensive Cancer Center).[74][75]

Schematickaryogramof a human, showing an overview of thehuman genome,with 22homologous chromosomes,both the female (XX) and male (XY) versions of thesex chromosome(bottom right), as well as themitochondrial genome(to scale at bottom left). The blue scale to the left of each chromosome pair (and the mitochondrial genome) shows its length in terms of millions of DNAbase pairs.
Further information:Karyotype

HGP scientists usedwhite blood cellsfrom the blood of two male and two female donors (randomly selected from 20 of each) – each donor yielding a separate DNA library. One of these libraries (RP11) was used considerably more than others, because of quality considerations. One minor technical issue is that male samples contain just over half as much DNA from the sex chromosomes (oneX chromosomeand oneY chromosome) compared to female samples (which contain twoX chromosomes). The other 22 chromosomes (the autosomes) are the same for both sexes.

Although the main sequencing phase of the HGP has been completed, studies of DNA variation continued in theInternational HapMap Project,whose goal was to identify patterns ofsingle-nucleotide polymorphism(SNP) groups (calledhaplotypes,or "haps" ). The DNA samples for the HapMap came from a total of 270 individuals;Yoruba peopleinIbadan,Nigeria;Japanese peopleinTokyo;Han ChineseinBeijing;and the FrenchCentre d'Etude du Polymorphisme Humain(CEPH) resource, which consisted of residents of the United States having ancestry from Western andNorthern Europe.

In the Celera Genomicsprivate-sectorproject, DNA from five different individuals were used for sequencing. The lead scientist of Celera Genomics at that time, Craig Venter, later acknowledged (in a public letter to the journalScience) that his DNA was one of 21 samples in the pool, five of which were selected for use.[76][77]

Developments[edit]

With the sequence in hand, the next step was to identify the genetic variants that increase the risk for common diseases like cancer and diabetes.[24][64]

It is anticipated that detailed knowledge of the human genome will provide new avenues for advances inmedicineandbiotechnology.Clear practical results of the project emerged even before the work was finished. For example, a number of companies, such asMyriad Genetics,started offering easy ways to administer genetic tests that can show predisposition to a variety of illnesses, includingbreast cancer,hemostasis disorders,cystic fibrosis,liverdiseases and many others. Also, theetiologiesforcancers,Alzheimer's diseaseand other areas of clinical interest are considered likely to benefit from genome information and possibly may lead in the long term to significant advances in their management.[78][79]

There are also many tangible benefits for biologists. For example, a researcher investigating a certain form ofcancermay have narrowed down their search to a particular gene. By visiting the human genome database on theWorld Wide Web,this researcher can examine what other scientists have written about this gene, including (potentially) the three-dimensional structure of its product, its functions, its evolutionary relationships to other human genes, or to genes in mice, yeast, or fruit flies, possible detrimental mutations, interactions with other genes, body tissues in which this gene is activated, and diseases associated with this gene or other datatypes. Further, a deeper understanding of the disease processes at the level of molecular biology may determine new therapeutic procedures. Given the established importance of DNA in molecular biology and its central role in determining the fundamental operation ofcellular processes,it is likely that expanded knowledge in this area will facilitate medical advances in numerous areas of clinical interest that may not have been possible without them.[80]

The analysis of similarities between DNA sequences from different organisms is also opening new avenues in the study ofevolution.In many cases, evolutionary questions can now be framed in terms ofmolecular biology;indeed, many major evolutionary milestones (the emergence of theribosomeandorganelles,the development ofembryoswith body plans, thevertebrateimmune system) can be related to the molecular level. Many questions about the similarities and differences between humans and their closest relatives (theprimates,and indeed the othermammals) are expected to be illuminated by the data in this project.[78][81]

The project inspired and paved the way for genomic work in other fields, such as agriculture. For example, by studying the genetic composition ofTritium aestivum,the world's most commonly used bread wheat, great insight has been gained into the ways that domestication has impacted the evolution of the plant.[82]It is being investigated which loci are most susceptible to manipulation, and how this plays out in evolutionary terms. Genetic sequencing has allowed these questions to be addressed for the first time, as specific loci can be compared in wild and domesticated strains of the plant. This will allow for advances in the genetic modification in the future which could yield healthier and disease-resistant wheat crops, among other things.

Ethical, legal, and social issues[edit]

At the onset of the Human Genome Project, several ethical, legal, and social concerns were raised in regard to how increased knowledge of the human genomecould be used to discriminate against people.One of the main concerns of most individuals was the fear that both employers and health insurance companies would refuse to hire individuals or refuse to provide insurance to people because of a health concern indicated by someone's genes.[83]In 1996, the United States passed theHealth Insurance Portability and Accountability Act(HIPAA), which protects against the unauthorized and non-consensual release of individually identifiable health information to any entity not actively engaged in the provision of healthcare services to a patient.[84]

Along with identifying all of the approximately 20,000–25,000 genes in the human genome (estimated at between 80,000 and 140,000 at the start of the project), the Human Genome Project also sought to address the ethical, legal, and social issues that were created by the onset of the project.[85]For that, the Ethical, Legal, and Social Implications (ELSI) program was founded in 1990. Five percent of the annual budget was allocated to address the ELSI arising from the project.[25][86]This budget started at approximately $1.57 million in the year 1990, but increased to approximately $18 million in the year 2014.[87]

Whilst the project may offer significant benefits to medicine and scientific research, some authors have emphasized the need to address the potential social consequences of mapping the human genome. Historian of scienceHans-Jörg Rheinbergerwrote that "the prospect of 'molecularizing' diseases and their possible cure will have a profound impact on what patients expect from medical help, and on a new generation of doctors' perception of illness."[88]

See also[edit]

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