Microscopic scale
Themicroscopic scale(fromAncient Greekμικρός(mikrós)'small' andσκοπέω(skopéō)'to look (at); examine, inspect') is the scale of objects and events smaller than those that can easily be seen by thenaked eye,requiring alensormicroscopeto see them clearly.[1]Inphysics,the microscopic scale is sometimes regarded as the scale between themacroscopic scaleand thequantum scale.[2][3]Microscopic units and measurements are used to classify and describe very small objects. One common microscopiclength scaleunit is themicrometre(also called amicron) (symbol: μm), which is one millionth of ametre.
History
[edit]Whilst compound microscopes were first developed in the 1590s, the significance of the microscopic scale was only truly established in the 1600s whenMarcello MalphigiandAntonie van Leeuwenhoekmicroscopically observed frog lungs and microorganisms. As microbiology was established, the significance of making scientific observations at a microscopic level increased.[4]
Published in 1665,Robert Hooke's book Micrographia details his microscopic observations including fossils insects, sponges, and plants, which was possible through his development of the compound microscope. During his studies of cork, he discovered plant cells and coined the term 'cell'.[5]
Prior to the use of the micro- prefix, other terms were originally incorporated into the Internationalmetric systemin 1795, such ascenti-which represented a factor of 10^-2, andmilli-,which represented a factor of 10^-3.[6]
Over time the importance of measurements made at the microscopic scale grew, and an instrument named the Millionometre was developed by watch-making company owner Antoine LeCoultre in 1844. This instrument had the ability to precisely measure objects to the nearest micrometre.[6]
TheBritish Association for the Advancement of Sciencecommittee incorporated the micro- prefix into the newly establishedCGS systemin 1873.[6]
The micro- prefix was finally added to the officialSI systemin 1960, acknowledging measurements that were made at an even smaller level, denoting a factor of 10^-6.[6]
Biology
[edit]By convention, the microscopic scale also includes classes of objects that are most commonly too small to see but of which some members are large enough to be observed with the eye. Such groups include theCladocera,planktonic greenalgaeof whichVolvoxis readily observable, and the protozoa of whichstentorcan be easily seen without aid. The submicroscopic scale similarly includes objects that are too small to see with anoptical microscope.[2]
Thermodynamics
[edit]Inthermodynamicsandstatistical mechanics,the microscopic scale is the scale at which we do not measure or directly observe the precise state of a thermodynamic system – such detailed states of a system are called microstates. We instead measure thermodynamic variables at amacroscopic scale,i.e. themacrostate.[citation needed]
Levels of Microscopic Scale
[edit]As the microscopic scale covers any object that cannot be seen by the naked eye, yet is visible under a microscope, the range of objects that fall under this scale can be as small as an atom, visible underneath atransmission electron microscope.[8]Microscope types are often distinguished by their mechanism and application, and can be divided into two general categories.[9]
Light microscopes
[edit]Amongst light microscopes, the utilisedobjective lensdictates how small of an object can be seen. These varying objective lenses can change the resolving power of the microscope, which determines the shortest distance that somebody is able to distinguish two separate objects through that microscope lens. It is important to note that the resolution between two objects varies from individual to individual,[9]but the strength of the objective lenses can be quantified.[11]
In the 1660s,Antonie van Leeuwenhoekdevised a simple microscope utilising a single spherical lens mounted between two thin brass plates. Depending on the quality of the lens, magnifications of between 70x and 250x were possible. The specimen to be examined was mounted on a point on a finely threaded rod.[12][13]
Compound light microscopeshave a short focal length objective lens which produces areal imagewhich is examined using a longer focal length eyepiece. The ratio of the focal length of the objective and the eyepiece, when mounted in a standard tube length, gives an approximate magnification of the system. Due to their design, compound microscopes have improved resolving power and contrast in comparison to simple microscopes,[11]and can be used to view the structure, shape and motility of a cell and its organisms,[14]which can be as small as 0.1 micrometres.[15]
Electron microscopes
[edit]While electron microscopes are still a form of compound microscope, their use ofelectronbeams to illuminate objects varies in mechanism significantly from compound light microscopes, allowing them to have a much higher resolving power, and magnification approximately 10,000 times more than light microscopes.[14]These can be used to view objects such asatoms,which are as small as 0.001 micrometres.[1]
Uses
[edit]Forensics
[edit]During forensic investigations,trace evidencefrom crime scenes such as blood, fingerprints and fibres can be closely examined under microscopes, even to the extent of determining the age of a trace. Along with other specimens, biological traces can be used to accurately identify individuals present at a location, down to cells found in their blood.[16]
Gemology
[edit]When the monetary value of gems is determined, various professions ingemologyrequire systematic observation of the microscopic physical and optical properties of gemstones.[17]This can involve the use of stereo microscopes to evaluate these qualities, to eventually determine the value of each individual jewel or gemstone.[18]This can be done similarly in evaluations of gold and other metals.[17]
Infrastructure
[edit]When assessing road materials, the microscopic composition of theinfrastructureis vital in determining the longevity and safety of the road, and the different requirements of varying locations. As chemical properties such as water permeability, structural stability andheat resistanceaffect the performance of different materials used in pavement mixes, they are taken into consideration when building for roads according to the traffic, weather, supply and budget in that area.[19]
Medicine
[edit]Inmedicine,diagnoses can be made with the assistance of microscopic observation of patientbiopsies,such as cancer cells.Pathologyandcytologyreports include a microscopic description, which consists of analyses performed using microscopes, histochemical stains orflow cytometry.These methods can determine the structure of the diseased tissue and the severity of the disease, and early detection is possible through identification of microscopic indications of illness.[21]
Microscopic scale in the laboratory
[edit]Whilst use of the microscopic scale has many roles and purposes in the scientific field, there are many biochemical patterns observed microscopically that have contributed significantly to the understanding of how human life relies on microscopic structures to function and live.[citation needed]
Founding experiments
[edit]Antonie van Leeuwenhoek was not only a contributor to the invention of the microscope, he is also referred to as the "father of Microbiology". This is due to his significant contributions in the initial observation and documentation ofunicellular organismssuch as bacteria and spermatozoa, and microscopic human tissue such as muscle fibres and capillaries.[22]
Biochemistry
[edit]Human cells
[edit]Genetic manipulation of energy-regulatingmitochondriaunder microscopic principles has also been found to extend organism lifespan, tackling age-associated issues in humans such asParkinson's,Alzheimer'sandmultiple sclerosis.By increasing the amount of energy products made by mitochondria, the lifespan of its cell, and thus organism, increases.[23]
DNA
[edit]Microscopic analysis of the spatial distribution of points withinDNAheterochromatincentromeresemphasise the role of the centromeric regions of chromosomes in nuclei undergoing theinterphasepart of cellmitosis.Such microscopic observations suggest nonrandom distribution and precise structure of centromeres during mitosis is a vital contributor to successful cell function and growth, even in cancer cells.[24]
Chemistry and physics
[edit]Theentropyand disorder of the universe can be observed at a microscopic scale, with reference to the second and thirdlaw of thermodynamics.In some cases, this can involve calculating the entropy change within a container of expanding gas molecules and relating it to the entropy change of its environment and the universe.[26]
Ecology
[edit]Ecologists monitor the state of an ecosystem over time by identifying microscopic features within the environment. This includes the temperature and CO2tolerance of microorganisms such as ciliates, and their interactions with othrt Protozoa. Additionally, microscopic factors such as movement and motility can be observed in water samples of that ecosystem.[27]
Geology
[edit]Branches ofgeologyinvolve the study of the Earth's structure at a microscopic level. Physical characteristics of rocks are recorded, and inpetrographythere is a specific focus on the examination of microscopic details of rocks. Similar to scanning electron microscopes, electron microprobes can be used inpetrologyto observe the condition that allows rocks to form, which can inform the origin of these samples. Instructural geology,petrographic microscopes allow the study of rock microstructures, to determine how geologic features such astectonic platesaffect the likelihood of earthquakes and groundwater movement.[28]
Current research
[edit]There have been both advances in microscopic technology, and discoveries in other areas of knowledge as a result of microscopic technology.[30]
Alzheimer's and Parkinson's disease
[edit]In conjunction with fluorescent tagging, molecular details in singularamyloidproteins can be studied through new light microscopy techniques, and their relation to Alzheimer's and Parkinson's disease.[31]
Atomic force microscopy
[edit]Other improvements in light microscopy include the ability to view sub-wavelength, nanosized objects.[32]Nanoscale imaging viaatomic force microscopyhas also been improved to allow a more precise observation of small amounts of complex objects, such ascell membranes.[33]
Renewable energy
[edit]Coherent microscopic patterns discovered in chemical systems support ideas of the resilience of certain substances againstentropicenvironments. This research is being utilised to inform the productions ofsolar fuels,and the improvement of renewable energy.[35]
Microscopic musical instrument - Micronium
[edit]A microscopic musical instrument called the Micronium has also been developed throughmicromechanics,consisting of springs the thickness of human hair being plucked by microscopic comb drives. This is a very minimal movement that produces an audible noise to the human ear, which was not previously done by past attempts with microscopic instruments.[36]
See also
[edit]- Macroscopic scale
- Microorganism
- Van Leeuwenhoek's microscopes
- Van Leeuwenhoek's microscopic discovery of microbial life (microorganisms)
References
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