- The DVD releases have new animation or removed dialog at the end of each chapter, which is where the respective Webisode faded to black while the actors usually ad-libbed.
- The Season 5 DVD removes some smaller jokes, including the last of Church's voice mail exchange (where he snarls "I! HATE! YOU! at the computer).
- The first five seasons were remade in the PC versions ofHalo: Combat Evolved (2001)andHalo 2 (2004)for the Blu-Ray box set containing the first ten seasons. Said remasters are also present in the official Red vs. Blue YouTube channel.
- On the DVD for Season 1, the music that plays on the Warthog is different than the music that played in the original downloadable episode. This change was done because the members of Red Vs Blue couldn't find who owned the rights to the song - they recorded it at a Mexican restaurant.
- The DVD version of Red Vs Blue Season 1 was given a new introduction mini-video. The style of this intro is repeated in Season 2 with Doc's character.
- Episode 36 ( "Nut. Doonut." ) was originally named "Dr. No-No" when it was released as a Webisode, and was changed to the current title for the DVD release, although different titles keep appearing when the episode is downloaded from redvsblue, including "Blunderball", "You Only Spawn Twice", and "Dummies are Forever".
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